377 Evaluation of the realquant® HBV-DNA assay (realtime PCR)We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco-2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 X 10...
Keywords:real-timefluorescencequantitativepolymerasechainreaction; DNA,hepatitisBvirus; linearrange; minimum detection“mjt 在本科室的实际工作中使用实时荧光定量PCR进行乙型 肝炎病毒(HBV)DNA检测的精密度、准确度,能长期、稳定地 控制在理想水平。各水平室内质控和各级室间质评都能很好 反应本实验室的精密度和准确...
performs fully automated real-time PCR amplification and detection, followed by interpretation of HBV DNA levels by means of Amplilink software. The manufacturer’s lower limit of detection (LOD) for the HBV DNA assay is 12 IU/ml (1.1 log 10 IU/ml), and the dynamic range of quantifi...
The quantitative PCR primers used are listed in Table 1. Table 1 The primers for real-time PCR Full size table Western blot analysis Cells were harvested, and the proteins were extracted with RIPA lysis buffer (1 mM MgCl2, 10 mM Tris–HCl pH 7.4, 0.1% SDS, 1% NP-40). Protein ...
timequantitiativePC1LTheresultsofdetetion bytwomethodswasthesamewhentheconcen~ationofHBVDNAwashigh.【Conclusion】 111ePEGprecipitate methodisthepraticalmethodofHBVDNAisolation.TaqmanrealtimePCRisthefirst-choise ofHBVDNAquanti- tiativeanalysis.SRBRrealtimePCRisasimple,economicalmethodofHBVDNAquanfifiati ...
these investigators detected cccDNA in peripheral blood mononuclear cells from highly viraemic, HBV-infected patients.[6] Real-time detection platforms increase the sensitivity of PCR assays compared with gel-based PCR assays, and \"real-time PCR\" is now considered the gold standard for selective...
DNA was purified by standard phenol chloroform extraction protocol and assayed by quantitative PCR on a CFX96 real-time PCR system (Bio-Rad) or LC96 (Roche) with Talent qPCR PreMix (SYBR Green, TIANGEN). Fold enrichment was calculated as percentage input and normalized to total H3. (For ...
The cDNAs were then used for real-time PCR (qPCR) on a CFX96 Touch Real-Time quantitative PCR System (Bio-Rad) using TB Green® Premix Ex Taq II (Tli RNaseH Plus; Takara). β-actin was served as the internal control. The relative quantification of gene expression was analyzed by ...
The qPCR was performed using FastStart Universal SYBR Green Master (Roche Applied Science) on a StepOne Real-Time PCR System (Applied Biosystems). As an internal control of extraction, in vitro-transcribed exogenous RNAs from the linearized Triplescript plasmid pTRI-Xef (Invitrogen) were added ...
Fingerprick whole blood capillary samples were tested using the SD BIOLINE HCV POC-RDT, Determine HIV Early Detect and Determine HBsAg 2, and the results were assessed against the LIAISON XL HCV, HIV and Murex-HBsAg-Quant, reference assays, respectively. The feasibility and user satisfaction of ...