[6] Zhang H,Ding J,Zhou Y.Quantitative PCR-based high-sensitivity detection of HBV-DNA levels reflects liver function deterioration in patients with hepatitis B virus-related cirrhosis.Am J Transl Res. 2024;16(6):2301-2309. [7] ...
HBV Quantitative Real Time PCR KitCat. No.: HD
目前检验病毒的方法,除去血清培养基方法(HIV, CMV, HBV等病毒很难在培养基中分离检验),大致有三种:PCR (polymerase chain reaction 或RT-PCR等),为最常用的方法,很敏感,但误差大,价钱比较贵,通常单位用 copies/ml;bDNA (branched chain DNA assay), 也是目前常用的方法,敏感,常规化,结果比较稳定,价钱比较便宜...
hepatitis B virus PCR detection assay is vital for the evaluation of the hepatitis b development stage and efficacy of antiviral treatment. In this article, we discuss the importance of the HBV DNA quantitative PCR test in assessing HBV infectivity and prescribing antivirus treatment for hepatitis...
并且应对HBV DNA“未检测到”和“HBV DNA存在但低于检测下限”两种结果模式进行区分,后者表示有PCR扩增曲线和痕量DNA存在,只是无法准确定量。此外也应考虑过分片面地强调HBV DNA检测的灵敏度,会导致污染带来的低值阳性结果被误当作LLV,从而给CHB的...
确定正常值指标一定要知道所做实验的 "参考范围" (reference range)。 参考范围是因测验的仪器,方法,试剂,病人年龄,性别,条件等而定的。 如,目前一般生产应用的Ultra Sensitive Quantitative PCR (高灵敏度定量PCR) 法的测验参考范围是在: Less than 0.001 pg/mL...
该文作者采用自动的、原型定量的(血清)HBV RNA检测方法(prototype quantitative HBV RNA assay),对未接受和接受NUC治疗的慢性HBV感染患者的循环HBV RNA(cirB-RNA)水平,以评估 cirB-RNA 浓度与血液中和肝细胞中的、与cccDNA 转录活性相关的其他病毒标物之间的关系,以评估其作为一种新的无创、可靠、可重复的、用于监...
14. Erhardt A, Schaefer S, Athanassiou N, et al: Quantitative assay of PCR-amplified hepatitis B virus DNA using a peroxidase-labelled DNA probe and enhanced chemiluminescence. J Clin Microbiol. 1996; 34: 1885 15. Moriyama K, Okamoto H, Tsuda F, et al: Reduced precore transcription and...
PCR-based assays are the most widely used method for HBV DNA quantification. A sensitive and accurate HBV DNA quantification assay is essential for monitoring hepatitis B virus (HBV) replication. Here are the Steps for HBV DNA Quantitative Detection:...
乙型肝炎病毒荧光PCR检测试剂盒(HBV)是基于荧光染料检测的即用型 PCR 试剂盒,可以对靶片段进行实时定量PCR 分析(quantitative PCR、qPCR)。产品含有经过优化的缓冲液组分、Taq DNA聚合酶、dNTPs、MgCl2及新型荧光染料 DNAgreen 等所有实时定量 PCR 所需要的成分,用户只需加入模板和引物即可进行实时定量 PCR 反应和 HRM...