Increasing the in vivo residence times of protein therapeutics could decrease their dosing frequencies. We show that genetic fusion of an unstructured recombinant polypeptide of 864 amino acids, called XTEN, to a peptide or protein provides an apparently generic approach to extend plasma half-life. ...
Increasing brain half-life of antibodies by additional binding to myelin oligodendrocyte glycoprotein, a CNS specific protein Abstract Background Therapeutic antibodies for the treatment of neurological disease show great potential, but their applications are rather limited due to limited brain exposure. The...
The protein-receptor complexes are recycled to the cell surface, where albumin and immunoglobulin molecules are released back into circulation at the neutral pH of the plasma. Several methods have been used to exploit the increased half-life of IgG or albumin for therapeutic payloads: (1) ...
The purpose of making a “biobetter” biologic is to improve on the salient characteristics of a known biologic for which there is, minimally, cl
Immunoglobulin (Ig)-binding proteins, mainly staphylococcal protein A (SPA) and streptococcal protein G (SPG), are commonly used as the ligand choice for antibody purification. More recently, the Ig-binding domains of SPA and SPG have been used to extend the serum half-life of small recombinant...
the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
half-lifeofDrp1inthepresenceofGFP-ParkinorGFPalonewasanalyzedby[ 35 S] methioninepulse-chaseassay,andtheproteinlevelswerequantified. FigureS2.InvitroubiquitinationofDrp1byMARCH5.InvitrotranslatedDrp1wasincubated withpurifiedubiquitin,E1,E2(UbcH7),variousParkinproteinsandimmunoprecipitated ...
Short half-life TKGFP fusion reporter gene for use in translational molecular-genetic imaging 来自 Semantic Scholar 喜欢 0 阅读量: 17 作者:C. Hsieh,Jung-Wen Kuo,Ren-Shyan Liu,S. Yeh 摘要: The low temporal resolution and cytototoxicity ofreporter protein of HSV1-tk/GFP (TKGFP) limited its...
Monoclonal IgG antibodies constitute the fastest growing class of therapeutics. Thus, there is an intense interest to design more potent antibody formats, where long plasma half-life is a commercially competitive differentiator affecting dosing, frequenc
B, the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein ...