Half life of the GFP variants in different ionic detergents.Sriram, SokalingamGovindan, RaghunathanNagasundarapandian, SoundrarajanSunGu, Lee
The protein-receptor complexes are recycled to the cell surface, where albumin and immunoglobulin molecules are released back into circulation at the neutral pH of the plasma. Several methods have been used to exploit the increased half-life of IgG or albumin for therapeutic payloads: (1) ...
Additional binding to MOG increases the Cmaxand brain half-life of antibodies that are actively shuttled over the BBB. Anti-SARS-CoV-2 antibodies coupled with an anti-TfR VHH and two low affinity anti-MOG VHHs could be detected in brain 49 days after a single intravenous injection, which i...
Monoclonal IgG antibodies constitute the fastest growing class of therapeutics. Thus, there is an intense interest to design more potent antibody formats, where long plasma half-life is a commercially competitive differentiator affecting dosing, frequenc
(100 μg/ml) for the indicated hours, and Western blotting was performed. B, the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP ...
thehalf-lifeofDrp1.A,293TcellsweretransfectedwithGFP-ParkinandGFP-Parkin(ΔR2) for24hours.CellswerethentreatedwithCHX(100μg/ml)fortheindicatedhours,and Westernblottingwasperformed.B,thelevelofremainingDrp1atdifferenttimepointswas quantifiedasthepercentageofinitialDrp1level(0hourofCHXtreatment).CandD,the...
Short half-life TKGFP fusion reporter gene for use in translational molecular-genetic imaging 来自 Semantic Scholar 喜欢 0 阅读量: 17 作者:C. Hsieh,Jung-Wen Kuo,Ren-Shyan Liu,S. Yeh 摘要: The low temporal resolution and cytototoxicity ofreporter protein of HSV1-tk/GFP (TKGFP) limited its...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...
While the first two rely on the increase of the hydrodynamic radius of the molecule of interest, the last two aim at diverting the natural FcRn dependent recycling of the albumin for the benefit of half-life extension [7]. Having a range of solutions at disposal is critical when considering...
The purpose of making a “biobetter” biologic is to improve on the salient characteristics of a known biologic for which there is, minimally, cl