1、鉴定的蛋白是直接作用的蛋白，非间接作用的复合体；2、GST标记可把一些难溶蛋白变成可溶蛋白，使实验变得更易操作；3、GSH谷胱甘肽偶联球珠亲和力强，洗脱纯度高。拉下实验（Pull-down）的缺点 1、两个非同一定位的蛋白可能因电荷或是强亲和力的原因造成假阳性，需用其他方法再次验证，例如Co-IP等；2、验证互作...
The GST pull-down assay is an intuitive and fast in vitro method for analyzing protein–protein or protein–ligand interactions and is comprised of a “bait” which is a GST-fused protein expressed in E. coli host or a baculovirus expression system and a “prey” which comprises putative bin...
Disease Research:GST Pull-Down assays can be applied to disease-related research. They can help identify aberrant protein-protein interactions associated with diseases such as cancer, neurodegenerative disorders, and infectious diseases. Screening for Binding Partners:Researchers can use GST Pull-Down assa...
GST Pull-Down Assays ServiceMany proteins function in association with partner proteins or as components of large multiprotein complexes. Understanding these protein interactions is critical to our understanding of biological pathways and cellular function....
Here, we detail using protein-protein interaction methods, including co-immunoprecipitation and Glutathione-S-transferase (GST) fusion protein pull-down assays to understand the molecular interactions of ATM. These assays give valuable functional insights into the role of ATM, as they are easy to ...
The following protocol provides general guidelines for GST pull-down assays using the MagneGST™ Pull-Down System and is based on methods we have developed using a MyoD/GST-Id protein pair. Optimization of protein- protein interaction and washing conditions may be required for each bait-prey ...
GST pull-down assays are extensively used to discover protein-protein interactions which involve in the af?nity puri?cations of one or more unknown...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your...
GST pull-down assays show other Lim-Homeodomain and POU-domain proteins interact with Pou4f2 and Isl1 respectively. 来自 figshare.com 喜欢 0 阅读量: 15 作者:R Li,F Wu,R Ruonala,D Sapkota,Z Hu,X Mu 年份: 2014 收藏 引用 批量引用 报错 分享 ...
Using sequence homology searches, yeast two-hybrid assays and glutathione -transferase (GST)-pull-down approaches we have identified a series of glutamate ... Hirbec,H. - 《Journal of Biological Chemistry》 被引量: 296发表: 2002年 PICK1 and Phosphorylation of the Glutamate Receptor 2 (GluR2)...
GST pull-down assays show other Lim-Homeodomain and POU-domain proteins interact with Pou4f2 and Isl1 respectively.Renzhong LiFuguo WuRaili RuonalaDarshan SapkotaZihua HuXiuqian Mu