Golden Gate Assembly (GGA) represents a versatile method for assembling multiple DNA fragments into a single molecule, which is widely used in rapid construction of complex expression cassettes for metabolic engineering. Here we describe the GGA method for facile construction and optimization of ...
The contigs corresponding to the repeat region of H-fibroin are not useful for foolproof assembly to obtain complete sequence, and do not harbour unique primer binding sites for amplifying partial or intermittent sequences18. AaFhc is rich in alanine codons (GCX), besides being a highly ...
为IIS克隆法(IIScloning),也称为“GoldenGate”克隆 法 [4] 。IIS型限制性内切酶能够特异识别双链DNA上 的靶位点,并在靶位点下游非特异性地对DNA双链进 行切割,在DNA双链的5′或3′端产生粘性末端 [5] 。 “GoldenGate”克隆法是在同一反应体系中,利用IIS型 ...
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GGAA plans to show at Portman Bay Mart. (Golden Gate Apparel Association)Marlow, Michael