Here, we cloned the β-galactosidase gene that encodes the glycoside hydrolase family 2 (GH2) enzyme LpGal2. Recombinant LpGal2 (rLpGal2, 72 kDa) is a homodimer with maximal enzymatic activity at pH 7.0 and 50 °C. Under these conditions, rLpGal2 hydrolyzes p -nitrophenyl-β-D-...
In molecular biology, Glycoside hydrolase family 2 is a family of glycoside hydrolases. Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification ...
Glycoside hydrolase family 2 signatures were retrieved from http://prosite.expasy.org/PDOC00531. For mul- tiple sequence alignments, ClustalW2 was employed (EMBL- EBI5, 2013). Hypothetical models of protein structures, based on sequence homologies, were prepared with the program "SWISS- MODEL" ...
Schwab C, Sorensen KI, Ga¨nzle MG (2010) Heterologous expression of glycoside hydrolase family 2 and 42 b-galactosi- dases of lactic acid bacteria in Lactococcus lactis. Syst Appl Microbiol 33:300-307Schwab C, Sorensen KI & G€anzle MG (2010) Heterologous expression of glycoside hydrolase...
Glycoside Hydrolase Family 2 作者: Jere, Wade Anastasia 页数: 72 ISBN: 9786200100658 豆瓣评分 目前无人评价 评价: 写笔记 写书评 加入购书单 分享到 推荐 我来说两句 短评 ··· 热门 还没人写过短评呢 我要写书评 Glycoside Hydrolase Family 2的书评 ··· ( 全部0 条 ) 论坛 ··· 在...
Glycoside hydrolases with a high degree of amino acid sequence homology can be classified in the same glycoside hydrolase (GH) family (Henrissat, 1991). Family members have the same catalytic mechanism (retaining or inverting) but can have different substrate specificity and/or mode of action. ...
In this work, the selectivity and modes of action of a glycoside hydrolase family active on β-1,3-glucans were systematically investigated combining sequence similarity network, phylogeny, X-ray crystallography, enzyme kinetics, mutagenesis and molecular dynamics. This family exhibits a minimalist and...
Trichoderma genus fungi present great potential for the production of carbohydrate-active enzymes (CAZYmes), including glycoside hydrolase (GH) family members. From a renewability perspective, CAZYmes can be biotechnologically exploited to convert plant
α-L-Fucosidases (EC 3.2.1.51), the only members of glycoside hydrolase family 29, are involved in many biological processes like inflammation, growth regulation, receptor interactions, and antigenicity. Their substrate specificity vitally depends on the enzyme source (bacteria, moulds, mollusks, pla...
Endo-β-1,3-glucanase GLU1, from the fruiting body of Lentinula edodes, belongs to a new glycoside hydrolase family. Sakamoto Y; Nakade K; Konno N.Endo- β-1,3 - glucanase GLU1 ,from the fruiting body of Lentinula edodes, belongs to a new glycoside hydrolase family.Applied and ...