The primary structure of the protein revealed that the catalytic module was highly similar to that of glycoside hydrolase family 27 (GH27) α-d-galactosidases. The recombinant protein was successfully expressed
Glucansucrases are classified as glycoside hydrolase (GH) enzymes of Henrissat's family 70.49 In terms of the EC-classification system, glucansucrases are designated as EC 2.4.1.5 (dextransucrase, but mutansucrase and reuteransucrase are still currently classified here) and EC 2.4.1.140 (alter...
Yang J, Zhang X, Yoon J, Rao K, Grate JH, Baidyaroy D, Elgart DD. February 2013. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity. US patent 20,130,052,698.Yang J, Zhang X, Yoon J, Rao K, Grate JH, Baidyaroy D, Elgart DD. February 2013. Gh...
www.nature.com/scientificreports OPEN A metagenome-derived thermostable β-glucanase with an unusual module architecture Received: 31 August 2017 Accepted: 16 November 2017 Published: xx xx xxxx which defines the new glycoside hydrolase family GH148 Angel Angelov1, Vu Thuy Trang Pham1, Maria Übe...
This article contains supplemental Figs. S1 and S2. 1 Both authors contributed equally to this work. 3 The abbreviations used are: GH18 glycoside hydrolase family 18 pNP 4-nitrophenolate ITC isothermal titration calorimetry MU methylumbelliferyl PDB Protein Data Bank. ...
Therefore, a glycoside hydrolase family 16 member, which encodes a putative endo-1,3(4)-β-glucanase, Crypa1.estExt_Genewise1.C_80859 (abbreviated to CpEng1), was presented in the Cluster 1 (Fig. 1), was selected for further analysis. The protein contained 287 amino acids and possessed...
α-Amylases are glycoside hydrolase enzymes that act on the α(1→4) glycosidic linkages in glycogen, starch, and related α-glucans, and are ubiquitously present in Nature. Most α-amylases have been classified in glycoside hydrolase family 13 with a typical (β/α)8-barrel containing two ...
In this study, through the analysis of all GH family members deposited in the CAZy database, protein structure classification information released by the CATH database, and further prediction of glycoside hydrolase structures with AlphaFold [22], we systematically investigated the complex sequence–...
Classification of Sph3 as a glycoside hydrolase containing conserved catalytic motifs (Fig. 2B) led to the creation of a new glycoside hydrolase family GH135. These results suggest that the spherulin 4 family, which until now had no known function, may embody this novel GH family. Author ...
Gerwig1, Stéphane Duboux2, Christina Vafiadi2, Tjaard Pijning3 & Lubbert Dijkhuizen1 Lactic acid bacteria possess a diversity of glucansucrase (GS) enzymes that belong to glycoside hydrolase family 70 (GH70) and convert sucrose into α-glucan polysaccharides with (α1 → 2...