Birch JR, Mainwaring DO, Racher AJ (2008) Use of the Glutamine Synthetase (GS) expression system for the rapid development of highly productive mammalian cell processes. In: Knäblein DJ (ed) Modern biopharmaceuticals: design, development and optimization. Wiley-VCH Verlag GmbH, Weinheim, ...
The invention relates to a glutamine synthetase expression vector which can be amplified and have two expression cassettes. The main components of the glutamine synthetase expression vector include the following six parts: a first expression cassette component, a second expression cassette component, an ...
Our observation that sarcomas were able to adapt and proliferate under glutamine deprivation raised the possibility that the cells may be synthesizing glutamine de novo to make up for the lack of exogenous glutamine, which depends on expression of the enzyme GS28,29,30. Increased de novo glutamine...
Glutamatergic synapses constitute a major excitatory neurotransmission system and are regulated by glutamate/glutamine (Gln) cycling between neurons and astrocytes. Gln synthetase (GS) produced by astrocytes plays an important role in maintaining the cycle. However, the significance of GS during synaptogene...
However,enzymatic activity of GS is influenced by many factors,leading to the low enzyme activity and the low production of L-Gln. In order to improve the yield of L-Gln,a high-effective expression system was constructed in Corynebacterium glutamicum with gln A gene. We tested two different ...
GLUTAMINE SYNTHETASE EXPRESSION MEDIUM SUPPLEMENT化学性质 储存条件: −20°C 形态: liquid 安全信息 WGK Germany:3 GLUTAMINE SYNTHETASE EXPRESSION MEDIUM SUPPLEMENT性质、用途与生产工艺 GLUTAMINE SYNTHETASE EXPRESSION MEDIUM SUPPLEMENT上下游产品信息 上游原料 ...
Glutamatetaken up by astroglial cells is either converted into glutamine byglutamine synthetase, which is exclusively located in glial cells (Hertz, 1979; Erecinska & Silver, 1990), or oxidized by assimilation into theKrebs cyclelocated in the mitochondria of glial cells. Once formed, the glu...
Glutamine is classified among non-essential amino acids since it can be synthesized from α-ketoglutarate, via glutamate dehydrogenase and glutamine synthetase; however, it becomes “conditionally essential” during cell proliferation and/or under specific illness conditions, especially in cancers [4]. ...
Glutamine stimulates the expression of the argininosuccinate synthetase () gene at both the level of enzyme activity and mRNA in Caco-2 cells. Searching to identify the pathway involved, we observed that (i) the stimulating effect of glutamine was totally mimicked by glucosamine addition, and (ii...
Changes in astrocyte glutamine synthetase (GS) in postischemic rat brain were evaluated and correlated with regional neuronal vulnerability or resistance t... CK Petito - 《Brain Research》 被引量: 286发表: 1992年 Cortical expression of glial fibrillary acidic protein and glutamine synthetase is dec...