Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. 蛋白标签: N-terminal 6xHis-SUMO-tagged 产品提供形式: Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you ...
Yeast DHODH and Aequorea GFPmut2 (1) coding sequences were inserted into anE. coli-Plasmodium falciparumshuttle plasmid (pHHMC*/3R0.5) (2), such that DHODH-GFP were fused into a single coding sequence (including a 10Ala-1Gly linker segment). ...
SequenceWith the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence. TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofect...
and 3D structures of the minimal Drp1 dimer (PDB ID: 4BEJ) and mEGFP (PDB ID: 2Y0G) drawn to scale. A Drp1 monomer is color-coded in correspondence with the above domain arrangement. The sequences of the linkers connecting mEGFP to Drp1 at the N- and C...
designed linker variant sequences. N.M. designed mammalian and protein S11 constructs; S.R.S. designed the protein-expressed GFP1-10 construct. M.N.S. performed construct cloning. B.A.C.L. validated SEE components, performed GFP1-10 immunoblotting and SEE complementation experiments in live ...
We therefore constructed an HDAd expressing GFP-tagged rabbit apoAI.Methods: We constructed HDAdGFPapoAI by fusing the GFP sequence to the N-terminus of the rabbit APOA1 gene with a short linker. Bovine aortic endothelial cells (BAEC) were transduced with HDAdGFPapoAI, washed, and incubated ...
2014). The linker sequence “W-T” was selected using an in-house random screening system. The cDNA fragments encoding the mature protein-coding regions were cloned into pET-23a vector (Novagen) to express the protein with a C-terminal His-tag. Protein expression and purification Escherichia co...
In the process they splice the flanking protein sequences, termed the N-and C-terminal exteins. Large inteins frequently have a homing endonuclease that is involved in maintaining the intein in the host. Splicing and nuclease activity are independent and distinct domains in the folded structure. ...
In a preferred from, the isolated mutant GFP has an amino acid sequence as set out in SEQ ID NO 4. In a fourth aspect, the present invention provides an unrepressed or constitutive gene cassette for providing a gene to a chromosome comprising an endogenous gene under the control of the ve...
by integrating a gene encoding a fluorescence labeling protein in E3 region of a viral genome and integrating a replication cassette comprising a human telomerase promoter, an E1A gene, an IRES sequence and an E1B gene in this order in E1 region, and then expressing both cassettes. Thus, the...