Size of EGFP Number of amino acids: 239Molecular weight (MW): 26,9 kDa Properties of EGFP Extinction coefficient (EC): 55,900 M-1 cm-1Maturation rate (at 37°C): 25 min EGFP sequences EGFP amino acid sequence: 10 20 30 40 50 MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDA...
徕卡双光子四维成像的斑马鱼胚胎的Expression of cytoplasmic GFP in the notochord and prechordal plate, 980nm. Nuclei stain through RNA injection H2B-mCherry @1030 nm. Courtesy of BioEmergences USR3695 (IBiSA FBI), Gif sur Yvettes, France. 连续33h的小鼠胚胎异染色质的形成需要关键的组蛋白变体H3.3...
(26.9kDa) and exhibits bright green fluorescence when exposed to ultraviolet blue light. It has become a well established reporter for gene expression and marker for protein targeting. Subtle modifications have been made to the sequence of GFP improving its solubility and photostablility (eGFP) to...
32、egfpS码序列上下游,实用文档EGFRS码序列存在特异的BanH I及Not I限制性内切酶位点,可切下整段10. pET-28a质粒全图谱pt1-2ito(小特qgg |看M1都科17 prnmnEer370_3GT7 LrjnsEiripLLon start30EUsrTsr coding s.BqyeFice270-287T7*Tafi CDdLrifi sequence207-239Multiple cloning sites (风?EH I 一...
'GFP and SceGFP3'. Repair of the I-SceI-generated DSB in SceGFP'3 by SSA results in a functional GFP gene when a DNA strand from SceGFP3' is annealed to the complementary strand of 5GFP', followed by appropriate DNA-processing steps. As a result, SSA between the homologous sequences...
Such proteins may be constructed by fusing C-terminal amino acids of the degradation domain of mouse ornithine decarboxylase (MODC), which contains a PEST sequence, to the C-terminal end of an enhanced variant of GFP (EGFP). Fluorescence intensity of the fusion protein in transfected cells is...
而用GFP就可以准确而简便地对肿瘤细胞进行示踪。 李侠【21】等将携有增强型绿色荧光蛋白(EGFP)基因的pEGFP-N3质粒体外转染C6胶质瘤细胞,筛选稳定表达绿色荧光蛋白的细胞克隆,以立体定向法植入SD大鼠脑实质内建立大鼠移植瘤模型。4周后处死大 17、鼠并作鼠脑连续石蜡切片,相邻的切片分别做苏木素-伊红(HE)或免疫组...
SpecificityThis antibody is reactive against all variants ofAequorea victoriaGFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP. Tested applicationsIHC-FoFr,Electron Microscopy,IHC-P,IHC-Fr,IP,WB,ICC/IF,IHC - Wholemount,Flow Cytmore details ...
pCAG-GFP质粒价格1200元,pCAG-GFP(addgene 11150 )质粒图谱(map),质粒序列(Sequence)见下文,质粒抗性为氨苄青霉素,质粒大小为4779 bp,测序引物为pCAG-F/EGFP-N。
将水母发光蛋白编码序列(由M.-T.Nicolas慷慨惠赠)以符合读码框的方式融合在EGFP基因的3’端,即插入pEGFP-C1的BgIII/SalI位点。为了在哺乳动物细胞中获得更好的表达,通过使用交叠延伸PCR(聚合酶链式反应)的定点诱变手段,更改了七个密码子。然后,将互补寡核苷酸5’-CCGGCGGGAGCGGATCCGGCGGCCAGT-3’[SEQ ID NO23...