The average insertion difference between the best codon variant and the worst was 13.3-fold, with the highest insertion rate for the cytosine-richest CAC histidine codons (Supplementary Fig. 10d). This directly demonstrates the practical utility of this new understanding for guiding the codon choice...
1a and Table 1). Thus, we decided to switch to R8 and eGFP as the model CPP and the model cargo, respectively. Different CPP sequences were cloned into the C-terminus of GFP in pEV eGFP plasmid (Fig. S6) that contains an inducible eGFP gene with an N-terminal His tag. Fusion ...
Despite the absence of a statistically significant difference fromk1, the only constructs other thank16that resulted in an increase of >5%on the fluorescence level ofk1werek3,k10, andk24. In particular, ink3, a thymine replaced an adenine at position −1, and ink10the adenine at position ...
HR leads to reconstitution of full-length EGFP coding sequence within green fluorescent RaDR-GFP pancreatic cells.Michelle, R. SukupJacksonOrsolya, KirályJennifer E., KayLi, NaElizabeth, A. RowlandKelly, E. WintherDanielle, N. ChowTakafumi, Kimoto...
The Forkhead (FKH) transcription factor FOXM1 is a key regulator of the cell cycle and is overexpressed in most types of cancer. FOXM1, similar to other FKH factors, binds to a canonical FKH motif in vitro. However, genome-wide mapping studies in differe
33). Altered nuclear staining, however, was the only apparent difference in localization patterns of the polybasic region mutants. As mentioned previously, various constructs with increasing numbers of polybasic point mutations were generated for both NTG14α- and NTG16α-EGFP-FLAG. These constructs ...
coli strains W and K-12 using the KIKO vectors. Expression levels varied between loci, with the arsB locus consistently showing the highest level of expression. To demonstrate the simultaneous use of all three loci in one strain, xynA, green fluorescent protein (gfp) and a sucrose catabolic ...
active in yeast, but inactive in mouse embryonic stem cells, consistent with a major difference in ‘default genomic states’ between these two divergent eukaryotic cell types, with implications for understanding pervasive transcription, horizontal transfer of genetic information and the birth of new ...
we calculated the distance between target genes and the nearest anti-phage defense gene. This distribution of distances was then compared with the distances between randomly selected genes and the nearest defense gene. The difference between these two distributions was calculated using a Wilcoxon rank-...
DX2 improves neuronal damage and behavior in rotenone-induced PD mouse model. FIG. 9D. The pole test. scAAV-DX2 recovered motor coordination and balance in the rotenone-treated PD mouse model. “Con” and “GFP” indicate wild type and rotenone-treated GFP injection mice. “Dose 1” and ...