Fluorophore and reagent selection guide for flow cytometry A handy reference poster featuring the broad range of our available dyes and labeling reagents. Request the guide Imaging protocol handbook Protocols that fit your needs in imaging ranging from sample and assay preparation to staining,...
100 µgELISA, IP, IHC, WB, Flow IgG * 2 mg/mL. # Immunoprecipitation (IP), immunohistochemistry (IHC), western blot (WB) detection, immunocytochemistry (ICC), and flow cytometry (Flow). TOP Materials The following protocol is designed for immunocy...
TOOLS FOR FLOW CYTOMETRY BioProbes 78 Flow cytometry compensation tools for a host of GFP variants GFP BrightComp eBeads Compensation Bead Kit. The discovery in the early 1960s [1] and sub- sequent development of Green Fluorescent Protein (GFP)...
This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high...
For other applications, we recommend the unconjugated version of this antibody, 66002-1-Ig 推荐稀释比 ApplicationDilution Immunofluorescence (IF)/ICC IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA) FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension It is recommended that th...
Flow cytometry analysis of transgene expression in higher plants: green-fluorescent protein Meth Cell Biol, 50 (1995), pp. 3-14 View PDFView articleView in ScopusGoogle Scholar [14] J Haseloff, B Amos GFP in plants Trends Genet, 11 (1995), pp. 328-329 View PDFView articleView in Scopus...
2b), and a small number of GFP-positive fibroblasts were detected by flow cytometry (Supplementary Fig. 7). We also observed that reconstituted-GFP signals were undetectable for several hours after the cancer cells supplying sC-GR migrated away from the interacting tissue-resident cells (...
pp 187–214 Cite this protocol Ion Channel Localization Bryan D. Moyer & Bruce A. Stanton Part of the book series: Methods in Pharmacology and Toxicology ((MIPT)) 574 Accesses Abstract The generation and maintenance of cell polarity is crucial for vectorial solute and fluid transport in ...
We thank Professor Greg Weiss for his advice and help with the manuscript, Dr Deborah Strickland for advice and help with flow cytometry experiments and “Bright Blue – the Police Commissioner’s Fund for Sick Kids” for their generous donation used to purchase the confocal microscope used in ...
Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, lower panel; Catalog #) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in GFP-positive cells. View our protocol forFluorescent ICC Staining of Cells on ...