flow cytometry demonstrated theexpression rates of RFP and GFP reached to 100%. The number of autophagic puncta significantly increased after starvationtreatment. Conclusion The RAW264.7 cell strain with stable expression of RFP-GFP-LC3 has been successfully constructed,which provides a reliable cellular...
In this chapter, we describe the utility of this probe for measuring autophagic activity in cultured cells by flow cytometry and for analyzing autophagic activity in zebrafish tissues by confocal microscopy.doi:10.1007/978-1-0716-1589-8_4Tomoya Eguchi...
Flow cytometry Assays are employed to more accurately assess the extent of LC3-II localization to the autophagosome in primary cells.