Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. It is a versatile biological marker for monitoring physiological pro...
FLOW cytometryGREEN fluorescent proteinINSECT baits & repellentsMOLECULAR cloningBackground: The wasabi receptor, also known as the Transient Receptor Potential Ankyrin 1 (TRPA1) ion channel, is a potential target for development of repellents for insects, like the pine weevi...
Ion Channel Localization Bryan D. Moyer & Bruce A. Stanton Part of the book series: Methods in Pharmacology and Toxicology ((MIPT)) 574 Accesses Abstract The generation and maintenance of cell polarity is crucial for vectorial solute and fluid transport in epithelial cells and for the normal...
2b), and a small number of GFP-positive fibroblasts were detected by flow cytometry (Supplementary Fig. 7). We also observed that reconstituted-GFP signals were undetectable for several hours after the cancer cells supplying sC-GR migrated away from the interacting tissue-resident cells (...
We thank Professor Greg Weiss for his advice and help with the manuscript, Dr Deborah Strickland for advice and help with flow cytometry experiments and “Bright Blue – the Police Commissioner’s Fund for Sick Kids” for their generous donation used to purchase the confocal microscope used in ...
The peripheral blood mononuclear cells were then analyzed by flow cytometry using a Becton-Dickinson FACScan (San Jose, CA). The leukocytes were gated on and fluorescence measured under the fluorescein isothiocyanate channel. All analysis and quantitation were performed using the Cyclops software from ...
Wide bandwidths are used where color images are captured and the sharp, bright green of GFP can be dis- tinguished from the broad-spectrum au- tofluorescence; narrower bandwidths are used only where quantitative fluorescence intensity measurements are made, such as in fluorimetric or flow ...
Functionally validated by Western blotting of NaV1.7 using anti-Sodium channel Nav1.7, clone N68/6 (Millipore, #MABN41) and Alkaline Phosphatase conjugated anti-mouse IgG. Also validated for NaV1.7 expression by intracellular flow cytometry (FACS) using anti-NaV 1.7 clone 68/6 and PE-labeled an...
Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. It is a versatile biological marker for monitoring physiological pro...
Cytofluorimetry for GFP was performed for 10,000 cells on an analytical device (Beckman Coulter, NaviosTM Flow Cytometer) using the 488 nm excitation laser and the FL1 emission channel (525/40 nm). Values collected included total cell number, gated cell number and geometric mean ...