The estimate of the DNA content obtained by flow cytometry enables to differentiate the fig cultivars.R. PioA.C.L. de OliveiraM. PasqualL.A.S. PioP.N. CuriR.B. BisiUniversidade Federal de Lavras (UFLA) Minas Gerais BrazilActa Horticulturae...
流式细胞检测实验方案(Protocol) 我们通常在50-100 μL的实验样品中使用0.5-1×106个细胞(一次测试)。由于应用不同,每个研究人员都应滴定试剂以获得最佳结果。 在抗体染色之前,用PBS冲洗细胞以去除血清蛋白。如果超过一种的抗体染色方案,需要使用正确的荧光染料组合。 所有孵育都应在冰上进行,并尽量减少光照。 样品...
Edu还可以很容易地与R-PE、R-PE串联染料和绿色荧光蛋白等荧光蛋白复合。 节选自:Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur. J. Immunol. 2019. 49: 1457–1973 欢迎转发到朋友圈,但谢绝复制粘贴转载 Promotions below...
1.外泌体简介 外泌体(Exosome)是由细胞分泌到胞外的30～150 nm的脂质双分子层囊泡,膜内包含了蛋白质、脂类、mRNA、miRNA和代谢物等,以及暴露在细胞外结构域的跨膜蛋白。外泌体是一种细胞间的通信系统,可以反映其来源细胞的生理或病理状态,用于病理、疾病诊断和药理研究。 图1. 外泌体组成示意图。 2.外泌...
参考文献:Daemen S, Chan MM, Schilling JD. Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH. STAR Protoc. 2021 Apr 29;2(2):100511. doi: 10.1016/j.xpro.2021.100511. PMID: 33997821; PMCID: PMC8102804....
Jackson AL, Warner NL. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose NR, Friedman H, Fahey JL, eds. Manual of Clinical Laboratory Immunology. 3rd ed. Washington, DC: American Society for Microbiolog...
Cell Proliferation Assays for Flow CytometryIntroduction Measuring a cell’s ability to proliferate is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. The most accurate method...
Cell Proliferation Assays for Flow Cytometry Introduction Measuring a cell’s ability to proliferate is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. The most accurate ...
Record cells by flow cytometry after acquiring the compensation beads. AM are double-positive for SiglecF and CD11c (Figures 2A-2C), and > 98% viable (Figure 2D). Figure 2. FACS analysis of BAL AM.A. Simple gating strategy for ...
for 40 min at room temperature (Keep in dark place).11. Wash and Centrifuge: Centrifuge at 1500-2000 rpm for 5 min, then wash cells once with 2 ml blocking buffer and centrifuge again at the same condition.12. FC analysis: Re-suspend cells in 1 x PBS and analyze on flow cytometry.