The inflamed and thickened skin frequently compromises the quality of single-cell suspensions generated from IMQ-induced skin lesions, which has an impact on subsequent analyses by flow cytometry. This protocol details the complete procedure for the establishment of a mouse model of psoriasis and flow...
Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population (Figure 1). The advantage of Click-iT® EdU labeling is that the small size of the dye azide allows for efficient ...
Cell Proliferation Assays for Flow CytometryIntroduction Measuring a cell’s ability to proliferate is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. The most accurate meth...
Flow cytometry is a powerful method, which is widely used for high-throughput quantitative and qualitative analysis of cells. However, its straightforward applicability for extracellular vesicles (EVs) and mainly exosomes is hampered by several challenges, reflecting mostly the small size of these vesic...
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
for 40 min at room temperature (Keep in dark place).11. Wash and Centrifuge: Centrifuge at 1500-2000 rpm for 5 min, then wash cells once with 2 ml blocking buffer and centrifuge again at the same condition.12. FC analysis: Re-suspend cells in 1 x PBS and analyze on flow cytometry.
Incubate secondary antibody at 4℃ for 30 min. Wash cells with PBS three times. Re-suspend cells with 500μl of PBS . Detection Keep the sample away from light and detect fluorescence signal as soon as possible with a flow cytometer....
参考文献:Daemen S, Chan MM, Schilling JD. Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH. STAR Protoc. 2021 Apr 29;2(2):100511. doi: 10.1016/j.xpro.2021.100511. PMID: 33997821; PMCID: PMC8102804....
Preservation of cells, choice of fixative, storage, and thawing conditions are recurrent issues for the analysis of phytoplankton by flow cytometry. We examined the effects of addition of the surfactant Pluronic F68 to glutaraldehyde-fixed photosynthetic organisms in cultures and natural samples. In pa...
Centrifuge at 1000 rpm for 10 minutes and aspirate supernatant. 4.Resuspend the cells to a concentration of 1 X 10e7/ ml (1 X 10e6/100μl). 5. Transfer 100μl cell suspension into each fresh tube. 6. Add 20μl of properly diluted antibody according to the protocol into the tubes ...