Whole blood flow cytometric analysis of platelets is increasingly utilized in recent years, primarily due to the sensitivity of this method, but also because it only requires a small amount of blood with minimal sample manipulation. We have developed a whole blood flow cytometry methodological ...
Whole blood flow cytometric analysis of platelets is increasingly utilized in recent years, primarily due to the sensitivity of this method, but also because it only requires a small amount of blood with minimal sample manipulation. We have developed a whole blood flow cytometry methodological ...
This protocol describes the immunophenotyping of cells in a whole blood sample by flow cytometry with minimal sample manipulation, thereby preserving cell structure and function while also reducing cell loss [1,2]. Whole blood samples st...
This protocol provides an overview of how to perform flow cytometric staining of whole blood samples, including steps on red blood cell lysis, surface marker staining, and fixation.
Focuses on the use of flow cytometry methods for counting of CD34 cells in blood. Use of single- or dual-laser excitation in the methods; Analysis of the cells in unlysed whole blood, stained with monoclonal antibodies; Identification of CD34 cells with class III monoclonal antibodies by ...
Isolation of mononuclear cells from human bone marrow aspirates by density gradient centrifugation (special protocol) 6 Sample preparation of blood Whole blood can be used directly for the isolation of target cells. In some cases, however, the isolation of a particular cell type c...
Westmead Hospital. Here we present a protocol for characterizing monocyte subsets by whole blood flow cytometry. This includes outlining how to gate the subsets and assess their expression of surface markers and giving an example of the assessment of th
In contrast to PBMCs, the use of a whole blood flow cytometry protocol enables the study of ASC speck formation in PMN; thus, we evaluated ASC speck formation in neutrophils using the current technique. The initial gating strategy for PMN identification was similar to monocytes (Figure 4A). ...
The present protocol describes in detail the experimental design and workflow to examine platelet reactivity and platelet maturity using whole blood flow cytometry. 2. Materials 2.1. Flow Cytometer All analyses were performed on a CytoFLEX S (B75442) flow cytometer (Beckman Coulter, Miami, FL, USA...
流式细胞术分析全血样本实验方案描述了在尽可能减少样本处理的情况下通过流式细胞术对全血样本进行免疫细胞分型。该方法不仅没有损害细胞结构和功能,而且能减少细胞损失[1,2]。可以直接在流式细胞仪上分析经细胞表面标志物抗体染色的全血样本。为方便...