Sickle cell anemia is caused by the mutation of a single base in the DNA sequence of the ß-globin gene, HBB. In healthy individuals, position 6 of the resulting amino acid sequence is a glutamic acid (GAG), however, in sickle cell anemia patients, this is substituted for a valine (...
Journal of Translational Medicine (2022) 20:535 Page 4 of 21 Finally, the delivery of genome editing nucleases through plasmids is an easy procedure. Due to the neces- sity of nuclear entry, subsequent transcription, and translation into protein, the genome editing efficacy is significantly ...
BFP, blue fluorescent protein; EF1a, elongation factor 1 alpha short variant promoter; EGFP, enhanced green fluorescent protein; IRES, internal ribosomal entry site; pA, polyadenylation site; SFFV, spleen focus forming virus promoter; SIN, self-inactivating long terminal repeat (LTR); U6, human ...
Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite its popula
They described that the CRISPR loci served as a self-defense mechanism in prokaryotes to prevent the entry of foreign genetic transmissible units [28,29,33,34]. In August 2005, another French group studying CRISPR spacers in the dairy bacterium Streptococcus thermophilus reported that the spacer...