RNA-seq was performed for either DO mice or mice of their progenitor strains (NOD/ShiLtJ as the AC-sensitive strain; 129S1/SvImJ as the AC-tolerant strain). For DO mice, 418 and 511 differentially expressed gene (DEGs) were specifically found in AC-sensitive and AC-tolerant mice,...
Overall, the primer sets targeting the hot-spot region did have a significance to detect majority of the beta-globin gene mutations. Table 3 The spectrum of HBB gene mutations in the regional hot-spot of South Asia and Southeast Asia Full size table Fig. 1 Schematic representation of ...
Full size image We employed the OP9 co-culture system to induce the hematopoietic differentiation of β-thalassemia iPSCs before and after gene correction. The morphologies of the TALENs-corrected iPSCs and the parental lines changed rapidly upon differentiation in OP9 co-culture (Supplementary Figur...