在本发明第一方面或第二方面中的一实施方式中,由引导序列和骨架序列组成的grna,可采用将grna对应的编码dna序列克隆到包含t7或sp6启动子的载体中(如pdr274,addgene),或通过pcr、退火、合成等方式直接在grna对应的编码dna的前端加上t7或sp6启动子,经转录可获得转录产物grna序列。在本发明第一方面或第二方面中的一...
it was found that the reduction in Hb oxygen-carrying capacity and the increase in DNA breakage are the two main pathways responsible for doxorubicin-induced cardiotoxicity, and the increased release of cf-Hb is a prerequisite for toxicity prior to DNA damage [...
Anaplastic thyroid cancer (ATC) is one of the most fulminant and foetal diseases in human malignancies. However, the genetic alterations and carcinogenic mechanisms of ATC are still unclear. Recently, we investigated the gene expression profile of 11 anaplastic thyroid cancer cell lines (ACL) and s...
20、ts on the HBB DNA sequences (NCBI Reference Sequence: NG_000007.3). Table 1 shows the primer sets for the detection of HBB gene mutations in promoter, exon 1, exon 2, intron 3 and exon 3. For the prenatal diagnosis of -thalassemia, we used primer P1+P3 for detecting the promoter...
Chemoresistance is a significant barrier to effective cancer treatment. Potential mechanisms for chemoresistance include reactive oxygen species (ROS) accumulation and expression of chemoresistance-promoting genes. Here, we report a novel function of lnc
Mutation-specific LV enhancement for HBBIVSI−110(G>A)-affected individuals may therefore be achieved by the expression of miRNA-embedded short hairpin RNA (shRNAmiR) sequences targeting HBBIVSI−110(G>A) mRNA with a view to reducing the potential interference of aberrant mRNA and to ...
The G→T change alters the DNA sequence for a key regulatory element ‘+62’ within the erythroid intronic enhancer for BCL11A [19]. This variant could be causal to HbF association seen with other closely-linked markers in the locus, such as rs766432, rs4671393 and rs1896296. Another SNP...