Su YN, Lee CN, Hung CC, Chen CA, Cheng WF, Tsao PN, Yu CL, Hsieh FJ: Rapid detection of beta-globin gene (HBB) mutations coupling heteroduplex and primer-extension analysis by DHPLC. Hum Mutat 2003, 22 (4) : 326-336. PubMed Abstract Publisher Full Text...
The sequences of ssODNs for gene correction are shown below. b Sanger sequencing chromatographs of the PCR amplicons of the wild-type HUDEP-2 and the HBB CD41-42 (-TCTT) mutant stable HUDEP-2 cell lines (HUDEP-2-CD41-42 M), with the homozygotes 4 bp ...
The genotyping of HBB gene was determined by polymerase chain reaction (PCR). The suitable primers were used for three exons of β-globin gene including the promoter, first intron, 5’ and 3’ untranslated region (UTR) sequences as previously reported [16]. For HBD gene, two specific ...
The full amino acid sequences of the TALENs are pro- vided in the supporting material (Supplementary Figure S1). CRISPR/Cas9 vector pX330 was purchased from Addgene (Ad42230). Guide RNAs were designed according to the rule of 5'-GN20NGG-3'. The guide RNA oligonucleotides were purchased ...
( pHBB) ~infusion~R CTTCCCCTTACTCGAGCAGGCATCATTATCAGTGAT 表 4 人源 hHBB 片段扩增及融合引物序列 Table 4 Primers used for PCR or infusion PCR of the human HBB gene 引物名称 Primer name 引物序列(5' -3') Primer sequence(5' -3' ) hHBB( Ex1-3) ~F1 hHBB( Ex1-3) ~R1 ...
Relationships of mRNA-protein secondary structures in the human -globin gene HBB and four variants. Chin Sci Bull, 2012, 57, doi: 10.1007/s11434-012-4996-3 2012 年 3 月第 57 卷第 7 期 液学上的表现与重型-地中海贫血相似, 可以出现 基因及其突变体 5 个样本中 mRNA-蛋白质二级结构...
Objective To use whole genome sequence data to determine whether these deletions arose from a single origin. Methods We used two approaches to confirm unrelatedness: pairwise comparison of SNPs and identity by descent analysis. Eagle, V.2.4, was used to generate phased haplotypes for the 683 ...
The lower steady-state amount of mRNA produced by the β-globinL1 allele also resulted from a reduced rate of transcription and decreased production of full-length β-globin primary transcripts. The promoter and enhancer sequences of the β-globinL1 allele were hypermethylated; however, treatment ...
The objective of this study was to develop an accurate and reliable method of sex identification in the yak. To achieve this goal, a duplex-nested polymerase chain reaction (PCR) was optimised using DNA from male and female yaks and primers specific for SRY and bHBB gene sequences. Males ...
Nonsense mutation of β‐globin gene at codon 82 (AAG→TAG) or HBB:C247 A→T with polymorphism: cause of thalassemia intermedia?Humansbeta-ThalassemiaCodonCodon, NonsenseBase SequenceHomozygotePolymorphism, Single NucleotideAdultChildFemaleNo abstract is available for this article.doi:10.1111/j.1751-...