During the last decade, fourstranded DNA structures known as Gquadruplexes, or DNA tetraplexes, have emerged as a threedimensional structure of special interest. Motifs for the formation of Gquadruplex DNA structures are widely dispersed in eukaryotic genomes, and are abundant in regions of ...
7,8. However, it is crucial to demonstrate the bona fide formation of G4 structures within an endogenous chromatin context9,10. Herein we address this through the development of G4 ChIP–seq, an antibody-based G4 chromatin
When the G4 structure was converted into the duplex conformation by addition of the complementary C-rich strand, the fluorophores were separated by ~150 Ǻ, and FRET was approximately 0. Treatment of LTR-II + III + IV G4 with hnRNP A2 increased fluorescence by 3-...
Structure of a G-quadruplex. Left: a G-tetrad. Right: an intramolecular G-quadruplex 3D Structure of the intramolecular human telomeric G-quadruplex in potassium solution (PDB ID 2HY9). The backbone is represented by a tube. The center of this structure contains three layers of G-tetrads. ...
The determined complex structure shows a 2:1 binding stoichiometry with each compound recruiting the adjacent flacking adenine residue to form a “quasi-triad plane” that stacks over the two external G-tetrads. The binding involves both π-stacking and electrostatic interactions. Moreover, berberine...
In solution, four d(TG4T) strands form a parallel-stranded G-quadruplex structure containing four stacked G-quartets in the presence of Na+ ions. This structure was also shown to be maintained when Na+ is replaced by K+. The crystal structures of the Na+ form of d(TG4T) show a ...
DNA G-Quadruplex Recognition In Vitro and in Live Cells by a Structure-Specific Nanobody. PMID: 36488193 Synthesis, conformation and cytotoxic activity of short hybrid peptides containing conformationally constrained 1-(aminomethyl)cyclohexanecarboxylic acid and gabapentin. PMID: 36279986 ...
G-quadruplex (G4) is a four-stranded helical DNA secondary structure formed by guanine-rich sequence folding, and G4 has been computationally predicted to exist in a wide range of species. Substantial evidence has supported the formation of endogenous G4 (eG4) in living cells and revealed its...
Fig. 1. G-quadruplex structure. (A) Structural representation of a G4 from human telomeric DNA (PDB: 1KF1) that folds into a parallel G4 structure (Parkinson, Lee, & Neidle, 2002). The guanines are represented as slabs and colored differently per quartet. G-strands are marked with...
RNA G-quadruplexes strongly enhanced mRNA stability, rather than affecting translation. Disruption of individual RNA G-quadruplex promotes mRNA decay in the cold, leading to impaired plant cold response. Therefore, we propose that plants adopted RNA G-quadruplex structure as a molecular signature to ...