EnhancerpromoterEpromotergene regulationreporter-assayCRISPR/Cas9Promoters with enhancer activity have been described recently. In this point of view, we will discuss current findings highlighting the commonality of this type of regulatory elements, their genetic and epigenetic characteristics, their potential...
Regulation of both transcription and RNA splicing requires enhancer elements, that is, cis -acting DNA or RNA sequences that promote the activities of linked promoters or splice sites, respectively. Both types of enhancer associate with regulatory proteins to form multicomponent enhancer complexes that...
Combinatorial Binding Leads to Diverse Regulatory Responses: Lmd Is a Tissue-Specific Modulator of Mef2 Activity. Gene expression is initiated through the action of transcription factors and their cofactors converging on enhancer elements leading to a defined activity. ... Cunha,Paulo,M.,... - 《...
To assess the functional role of gene-distal enhancers, we devised the dCas9-based enhancer-targeting epigenetic perturbation systems for targeted modulation of enhancer activity in situ. Specifically, we employed the structure-guided sgRNA design by adding two MS2 hairpins, which is recognized by th...
Now we summarize the role of basic region/leucine zipper protein family members and particularly the albumin D site-binding protein (DBP) and the CAAT/enhancer-binding proteins (C/EBPs) for their importance in liver-specific gene expression and their role in liver function and development. ...
We find substantial genetic evidence to support the concept that lineage-determining transcription factors define epigenetic and transcriptomic states by selecting enhancer-like regions in the genome in a collaborative fashion and facilitating binding of signal-dependent factors. This hierarchical model of ...
(via increased NO availability; Figure 1), (ii) reducing oxidative stress (by acting as an antioxidant, e.g., as a ROS scavenger or enhancer of endogenous antioxidant capacity; Figure 2), and (iii) inhibiting the production of pro-inflammatory cytokines to prevent excessive post-exercise ...
The neuron-restrictive silencer factor (NRSF) is a major transcriptional repressor of the MOR promoter that has a critical role in the regulation of the MOR gene and a synergic repression effect through its interaction with the Sp3 transcription factor [10], [11]. NRSF was originally identified...
In the intestine, Notch signaling, especially Notch1, regulates stem cell-fate determination in the crypt [14]. Once stimulated, activation of Notch upon ligand binding promotes the expression of Hes-1 (Hairy and Enhancer-of-split-1), which then would suppress downstream Math1, resulting in ...
Genetic dissection of the α-globin super-enhancer in vivo. Nat. Genet. 48, 895–903 (2016). CAS PubMed PubMed Central Google Scholar Siersbæk, R. et al. Transcription factor cooperativity in early adipogenic hotspots and super-enhancers. Cell Rep. 7, 1443–1455 (2014). PubMed ...