et al. An enhancer required for transcription of the Col6a1 gene in muscle connective tissue is induced by signals released from muscle cells. Exp. Cell Res. 314, 3508-3518 (2008).Braghetta P, Ferrari A, Fabbro C, Bizzotto D, Volpin D, Bonaldo P, Bressan GM 2008. An enhancer required...
Here we use complimentary biochemical, genomic and structural approaches to test whether LFY is a bonafide pioneer transcription factor. We find that in vitro LFY binds with high affinity and specificity to a native regulatory fragment from theAP1locus in the context of a nucleosome. In vivo, th...
This is evidence for stable nucleosome binding by a transcription factor and shows that a winged helix protein is sufficient to initiate the assembly of an enhancer complex on nonacetylated nucleosomes. 展开 关键词: Animals Mice Chromatin Nucleosomes Escherichia coli Transcription Factors DNA DNA-...
Gene regulation during development depends on the coordinated action of enhancer and promoter sequences. Enhancers respond to specific developmental signals and transmit the information to the core promoter where transcription of the gene begins1. Since promoters must respond to a large variety of differ...
The nucleolar transcription factor mUBF is phosphorylated by casein kinase II in the C-terminal hyperacidic tail which is essential for transactivation. UBF is a DNA binding protein which interacts with both the promoter and the enhancer of various vertebrate ribosomal RNA genes and functions as a...
The transcription factor C/EBP alpha, a member of the CCAAT/enhancer-binding protein family, is highly expressed in the liver and in adipose tissue. The aim of this study was to determine if C/EBP alpha is expressed in rat growth cartilage. The expression pattern of C/EBP alpha in monolay...
In a hierarchical model of enhancer function, binding of one initiating TF may displace nucleosomes and expose binding sites for other secondary TFs (Iwafuchi-Doi and Zaret, 2014). Alternatively, in a billboard model, multiple TFs may independently bind to an enhancer, and any one TF may ...
We have demonstrated that the vasoactive intestinal peptide regulatory element is an enhancer that depends upon the integrity of two CGTCA sequence motifs for biological activity. Mutations in either of the CGTCA motifs diminish the ability of the element to respond to cAMP. Enhancers containing the...
TEAD1 (TEA domain transcription factor 1), a transcription factor known for the functional output of Hippo signaling, is important for tumorigenesis. However, the role of TEAD1 in the development of vascular smooth muscle cell (VSMC) is unknown. To investigate cell-specific role of Tead1, we...
MusTRD1 was identified by O'Mahoney et al23 as a protein that binds to an enhancer involved in the regulation of skeletal muscle gene expression. They identified the sequence elements (E-box, MEF2, USE B1 and USE C1) of the human troponin I slow upstream enhancer (USE) that bind muscle...