5. Sun, M., and Zhang, X. (2022). Current methodologies in protein ubiquitination characterization: from ubiquitinated protein to ubiquitin chain architecture. Cell Biosci. 12, 126.6. Leestemaker, Y., and Ovaa, H. (2017). Tools to investigate the ubiquitin proteasome system. Drug Discov....
Proteins of interest also formed tetramers by fusing to the inducible tetramer formation system, which exhibits its utility in a broad range of biological applications. 展开 关键词: FRB FKBP Rapamycin Oligomer Chemically-induced oligomerization Protein engineering DOI: 10.1016/j.jbiosc.2015.12.004 ...
A chemically inducible activation system for Fas (CD95/APO-1), which works efficiently to induce apoptosis and leads non-apoptotic pathways, has not yet been developed. Here, we engineered a rapamycin-induced dimerization system of Fas consisting of FKBP and FRB proteins. Treatment of rapamycin ...
摘要: •Fusion proteins containing FRB and FKBP formed oligomers on adding rapamycin.•Various oligomers were generated by adjusting the configuration of fusion proteins.•FRB–FKBP fusion protein without a linker (FR–FK) specifically formed a tetramer.•Proteins fused to FR–FK also formed ...
By adjusting the configuration of fusion proteins, we succeeded in generating an inducible tetramer formation system. Proteins of interest also formed tetramers by fusing to the inducible tetramer formation system, which exhibits its utility in a broad range of biological applications.Tomonao Inobe...