之后，研究人员引入Rapa诱导的FKBP·FRB系统，分别构建了NUb-FRBE3(63)和FKBP-CUbPCNA融合蛋白，体外实验同样证实，Rapa诱导能够促进底物的K63链接多聚泛素化修饰。同时，为了降低由Nub和CUb之间亲和性引起的背景泛素化活性，研究人员在NUb中引入了一个点突变I13A，这个突变质粒标记为NUa，并将融合质粒NUa-HA-FRB称为...
We previously advanced an inducible dimerization system into an inducible oligomerization system by developing a bivalent fusion protein, FRB–FKBP, which forms large oligomers upon rapamycin addition and can be used to manipulate cells. However, the oligomeric structure of FRB–FKBP remains unclear. ...
想要用BRET方法来鉴定FKBP12与FRB的相互作用。但是看得文献上都是需要共转至细胞里进行实验。有没有人...
Label-free Single-Molecule Quantification of Rapamycin-induced FKBP–FRB Dimerization for Direct Control of Cellular Mechanotran Subscriber access provided by Macquarie Universityis published by the American Chemical Society. 1155 Sixteenth Street N.W.,Washington, DC 20036Published by American Chemical Socie...
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research papers Re 庐 ned structure of the FKBP12 卤 rapamycin 卤 FRB 脢 resolution ternary complex at 2 . 2 A research papersLiang, JunChoi, JungwonClardy, Jon
Inobe, T. & Nukina, N. Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins. J. Biosci. Bioeng. 122, 40-46 (2016).Inobe, T., and Nukina, N. (2016). Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins. J. Biosci. Bioeng. 122, 40-46. ...
•Fusion proteins containing FRB and FKBP formed oligomers on adding rapamycin.•Various oligomers were generated by adjusting the configuration of fusion proteins.•FRB–FKBP fusion protein without a linker (FR–FK) specifically formed a tetramer.•Proteins fused to FR–FK also formed a tetram...
Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins Most proteins form larger protein complexes and perform multiple functions in the cell. Thus, artificial regulation of protein complex formation controls t... T Inobe,N Nukina - 《Journal of Bioscience & Bioengineering》 被引量...