之后,研究人员引入Rapa诱导的FKBP·FRB系统,分别构建了NUb-FRBE3(63)和FKBP-CUbPCNA融合蛋白,体外实验同样证实,Rapa诱导能够促进底物的K63链接多聚泛素化修饰。同时,为了降低由Nub和CUb之间亲和性引起的背景泛素化活性,研究人员在NUb中引入了一个点突变I13A,这个突变质粒标记为NUa,并将融合质粒NUa-HA-FRB称为...
FRBFKBPRapamycinOligomerChemically-induced oligomerizationProtein engineeringMost proteins form larger protein complexes and perform multiple functions in the cell. Thus, artificial regulation of protein complex formation controls the cellular functions that involve protein complexes. Although several artificial ...
The structure of the FKBP12–rapamycin–FRB ternary complex has now been refined at 2.2 Å resolution. The cell-cycle arrest agent rapamycin binds FK506-binding protein (FKBP12) and the FKBP12–rapamycin binding (FRB) domain of FKBP12–rapamycin associated protein (FRAP) simultaneously, and...
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research papers Re 庐 ned structure of the FKBP12 卤 rapamycin 卤 FRB 脢 resolution ternary complex at 2 . 2 A research papersLiang, JunChoi, JungwonClardy, Jon
联系人:Dr.Xu, Biovector NTCC Inc. 电话:400-800-2947 工作QQ:1843439339 (微信同号) 邮件:Biovector@163.com 手机:18901268599 地址:北京 已注册 详细信息 咨询记录(共0条) Plasmid Name质粒名称:BioVector® pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett plasmid...
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In this study, we showed that fusion proteins comprising the induced heterodimer formation proteins FRB and FKBP formed various oligomers upon addition of rapamycin. By adjusting the configuration of fusion proteins, we succeeded in generating an inducible tetramer formation system. Proteins of interest ...
摘要: •Fusion proteins containing FRB and FKBP formed oligomers on adding rapamycin.•Various oligomers were generated by adjusting the configuration of fusion proteins.•FRB–FKBP fusion protein without a linker (FR–FK) specifically formed a tetramer.•Proteins fused to FR–FK also formed ...