Essential to its various functions is its ability to bind simultaneously to two different proteins, FKBP and mTOR. Despite its widespread use, a thorough analysis of the interactions between FKBP, rapamycin, and the rapamycin-binding domain of mTOR, FRB, is lacking. To probe the affinities ...
FKBP12-rapamycin复合物的结合位点(FKBP12-rapamycin binding,FRB)为雷帕霉素(rapamycin,RAP)与哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)结合的结构域,基于RAP介导FK506结合蛋白12(12 kD FK506-bin-ging protein,FKBP12)与FRB蛋白质相互作用相关研究技术的发展与应用,使人们对小分子介导的蛋白质相...
FRB结构域即FKBP12-rapamycin复合物的结合位点(FKBP12-rapamycin binding,FRB),为mTOR氨基端2 015-2 114氨基酸残基之间的结构,是雷帕霉素(rapamycin,RAP)产生抑制mTOR活性的结合位点。RAP部分结构(C1-C15和C32-C41)与FK506结合蛋白12(12 kD FK506-binging protein,FKBP12)结合形成二元(RAP-FKBP12)复合物,药理...
Structure of the FKBP12-Rapamycin Complex Interacting with Binding Domain of Human FRAP A crystal structure of the ternary complex of human FKBP12, rapamycin, and the FKBP12-rapamycin-binding (FRB) domain of human FRAP at a resolution of... J Choi,J Chen,SL Schreiber,... - 《Science》 ...
Alternative Name: mTOR (human FKBP12-rapamycin-binding domain)Host: RabbitImmunogen: Recombinant human mTOR (FKBP12-rapamycin-binding (FRB) domain). UniProt ID: P42345 Application: Chromatin Immunoprecipitation Immunocytochemistry Western Blot (1:2’000) Specificity: Recognizes the FRB domain of fusion...
Chaurasia, S., Pieraccini, S., De Gonda, R., Conti, S. & Sironi, M. Molecular insights into the stabilization of protein-protein interactions with small molecule: The FKBP12-rapamycin-FRB case study. Chemical Physics Letters. 587, 68-74, doi: 10.1016/j. cplett.2013.09.042 (2013)....
•Fusion proteins containing FRB and FKBP formed oligomers on adding rapamycin.•Various oligomers were generated by adjusting the configuration of fusion proteins.•FRB–FKBP fusion protein without a linker (FR–FK) specifically formed a tetramer.•Proteins fused to FR–FK also formed a tetram...
Two modes of direct small-molecule inhibition of mTOR activity are known: targeting of the kinase active site and a unique mode in which the small molecule rapamycin, in complex with FKBP12 (the 12-kDa FK506 binding protein), binds to the FRB (FKBP12/rapamycin binding) domain of mTOR and...
Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins Most proteins form larger protein complexes and perform multiple functions in the cell. Thus, artificial regulation of protein complex formation controls t... T Inobe,N Nukina - 《Journal of Bioscience & Bioengineering》 被引量...