想要用BRET方法来鉴定FKBP12与FRB的相互作用。但是看得文献上都是需要共转至细胞里进行实验。有没有人...
想要用BRET方法来鉴定FKBP12与FRB的相互作用。但是看得文献上都是需要共转至细胞里进行实验。有没有人...
Choi, J. Clardy, Refined structure of the FKBP12-rapamycin-FRB ternary complex at 2.2 A resolution, Acta Crystallogr. D 55 (1999) 736-744.Liang, J.; Choi, J.; Clardy, J. Refined structure of the FKBP12- rapamycin-FRB ternary complex at 2.2 A resolution. Acta Crystallogr...
The structure of the FKBP12–rapamycin–FRB ternary complex has now been refined at 2.2 Å resolution. The cell-cycle arrest agent rapamycin binds FK506-binding protein (FKBP12) and the FKBP12–rapamycin binding (FRB) domain of FKBP12–rapamycin associated protein (FRAP) simultaneously, and...
research papers Re 庐 ned structure of the FKBP12 卤 rapamycin 卤 FRB 脢 resolution ternary complex at 2 . 2 A research papersLiang, JunChoi, JungwonClardy, Jon
Here we consider a typical case of protein-protein interaction stabilization: the complex between FKBP12 and FRB with rapamycin. We have analyzed the stability of the complex and characterized its interactions at the atomic level by performing free energy calculations and computational alanine scanning....
想要用BRET方法来鉴定FKBP12与FRB的相互作用。但是看得文献上都是需要共转至细胞里进行实验。有没有人...