之后，研究人员引入Rapa诱导的FKBP·FRB系统，分别构建了NUb-FRBE3(63)和FKBP-CUbPCNA融合蛋白，体外实验同样证实，Rapa诱导能够促进底物的K63链接多聚泛素化修饰。同时，为了降低由Nub和CUb之间亲和性引起的背景泛素化活性，研究人员在NUb中引入了一个点突变I13A，这个突变质粒标记为NUa，并将融合质粒NUa-HA-FRB称为...
Label-free Single-Molecule Quantification of Rapamycin-induced FKBP–FRB Dimerization for Direct Control of Cellular Mechanotran Subscriber access provided by Macquarie Universityis published by the American Chemical Society. 1155 Sixteenth Street N.W.,Washington, DC 20036Published by American Chemical Socie...
The present study exemplifies the PPI in vitro inside a unique single-chain probe, named TP2.4, which carries a full-length artificial luciferase (ALuc) sandwiched in between two model proteins of interest, e.g., FKBP and FRB, expressed in E. coli, and purified. We found that the TP2.4...
想要用BRET方法来鉴定FKBP12与FRB的相互作用。但是看得文献上都是需要共转至细胞里进行实验。有没有人...
PSF3-FLAG-CBIR-FRB_MYC-NBIR-FKBP CBNumber: CB513280513 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 PSF3-FLAG-CBIR-FRB_MYC-NBIR-FKBP化学性质 安全信息 PSF3-FLAG-CBIR-FRB_MYC-NBIR-FKBP性质、用途与生产工艺
联系人:龚思雨 联系电话:17801761073010-50973130 联系邮箱:3193328036@qq.com 网站:https://www.solarbio.com QQ:3193328036 微信: 产品规格 规格编号规格库存价格 VT033476-1EA1株现货面议 产品说明 索莱宝直销,欢迎咨询 相关产品 pSF3-Flag-CBir-FRB_Myc-NBir-FKBP...
research papers Re 庐 ned structure of the FKBP12 卤 rapamycin 卤 FRB 脢 resolution ternary complex at 2 . 2 A research papersLiang, JunChoi, JungwonClardy, Jon
We previously advanced an inducible dimerization system into an inducible oligomerization system by developing a bivalent fusion protein, FRB–FKBP, which forms large oligomers upon rapamycin addition and can be used to manipulate cells. However, the oligomeric structure of FRB–FKBP remains unclear. ...
Here, we engineered a rapamycin-induced dimerization system of Fas consisting of FKBP and FRB proteins. Treatment of rapamycin specifically induces cellular apoptosis. In neurons and cells with high c-FLIP expression, rapamycin-induced Fas activation triggered the activation of the non-apoptotic pathway...
想要用BRET方法来鉴定FKBP12与FRB的相互作用。但是看得文献上都是需要共转至细胞里进行实验。有没有人...