同时，为了实现可诱导性，研究人员引入了FKBP·FRB系统，在rapamycin诱导的情况下，FKBP和FRB结构域能够结合在一起。这样，当NUb和CUb分别与FKBP和FRB融合时，在rapamycin诱导的情况下，通过FKBP和FRB的结合，NUb和CUb也会相应的结合在一起形成一个完整的泛素分子。另外，根据链接方式的不同，如果是研究K48和K63链接...
FKBP-DTag技术的基本原理是利用FKBP和FRB之间的结合能力,将FKBP和FRB标记在需要研究的蛋白质上,并通过添加小分子化合物Rapamycin来诱导FKBP和FRB的结合。Rapamycin是一种免疫抑制剂,能够与FKBP和FRB形成三聚体复合物。当FKBP和FRB标记的蛋白质分别与Rapamycin结合时,它们会相互吸引并形成复合物,从而实现蛋白质的相互作用。
Wandless, T J
mTOR is a ∼270- to 290-kDa protein, which, going from the N toward the C terminus, consists of two clusters of ∼20 random repeats called HEAT motifs, a ∼500 amino acid FAT domain, an FKBP-rapamycin-binding (FRB) domain, the kinase domain, and, most C-terminally, a ∼35 ...
Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins Most proteins form larger protein complexes and perform multiple functions in the cell. Thus, artificial regulation of protein complex formation controls t... T Inobe,N Nukina - 《Journal of Bioscience & Bioengineering》 被引量...
or worksproduced by employees of any Commonwealth realm Crown government in thecourse of their duties.CommunicationLabel-free single-molecule quantification of rapamycin-induced FKBP-FRB dimerization for direct control of cellular mechanotransductionYinan Wang, Samuel Barnett, Shimin Le, Zhenhuan Guo,Xueying...
research papers Re 庐 ned structure of the FKBP12 卤 rapamycin 卤 FRB 脢 resolution ternary complex at 2 . 2 A research papersLiang, JunChoi, JungwonClardy, Jon
(Fig.2f). As with the FKBP52-containing structure, no GR prolines appear to be isomerized and the PPIase inhibitors rapamycin and FK506 sterically clash with the GR backbone. Interestingly, the small FKBP51-specific inhibitor, SAFit2 (PDB ID:6TXX)35,57, does not clash with the GR ...
We also successfully demonstrated the visualization of the rapamycin-mediated complexation of the OG-FKBP12 and FRB inside of living cells by the combined use with fluorescent protein-tag technology and Frster resonance energy-transfer imaging. 展开 ...
FK1 domain is active as PPIase and known to bind to the FK506 and rapamycin inhibitors or modulators36. The TPR domain is protein-protein interaction site with a concave surface which hosts the C-terminal peptide from Hsp90. Figure 2 Crystal structure of FKBP51-Hsp90 C-terminal peptide ...