Foxp3-mediated regulation unique to the thymus affects, among others, genes encoding nuclear factors that control gene expression and chromatin remodelling. In contrast, Foxp3 target genes shared by the thymic and peripheral T_R cells encode primarily plasma membrane proteins, as well as cell ...
Target Protein:FoxP3 IR:Immunogen Range:11-100/431 Clonality:Polyclonal Isotype:IgG Entrez Gene:50943 Swiss Prot:Q9BZS1 Source:KLH conjugated synthetic peptide derived from human FoxP3:11-100/431 Purification:affinity purified by Protein A Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin30...
Full size image These intra-rung mutations disrupted cellular functions of FOXP3, as measured by FOXP3-induced gene expression (for example, CTLA4 and CD25 protein levels (Fig.3d) and genome-wide mRNA levels (as measured by FOXP3 mRNA-seq in Extended Data Fig.5a)), target loci binding (a...
autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to ...
Target Information FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25...
Application Created with Sketch. 图:1/10 FOXP3 Antibody (700914) in IHC (P) Immunohistochemistry analysis of FOXP3 showing staining in the nucleus of paraffin-embedded mouse spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen ...
Target Protein FOXP3 Clonality Monoclonal Storage/Stability -20°C/1 year Application WB,IHC-p,IF,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. Concent...
Each antibody in this panel recognizes endogenous levels of its specific target protein. Source / Purification Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PD-L1 protein, recombinant ...
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Volume (to add to vial) ...
f, Distribution of perturbed gene eigencentrality calculated on either the complete target regulatory network graph (top) or a subset (bottom, edges with an absolute coefficient ≥ 0.2, related to (e)). g, Volcano plot of differentially regulated surface proteins between FOXP3-expressing RBPJ KO...