The recognition of specific aberrant patterns of leucocyte differentiation antigens on leukemic cells now allows flow cytometry to be used as a tool for assessing treatment response in acute myeloid leukaemia (AML), based on detection of small numbers of abnormal cells surviving therapy, therefore ...
Flow cytometry is an exact technology, INTRODUCTION: Immunophenotyping by flow cytometry offers a rapid, precise and effective means for the diagnosis of acute myeloid leukaemia (AML). It provides valuable information of myeloid lineage assignment of the leukaemic blasts. More than 98% of acute ...
首先, AML 是一种造血干细胞 (HSC) 的疾病, 其特点是: 停止分化, 随后聚集白血病爆炸细胞, 减少功能性造血元件的生产。异质性延伸到白血病干细胞的存在, 这一动态细胞室的演变, 以克服各种选择压力强加在白血病的进展和治疗。为了进一步定义非定常种群, 添加 CD90 和 CD45RA 允许在 CD34+CD38 细胞间...
A flow cytometry method has been introduced into the routine investigation of whole bone marrow samples following red blood cell lysis on the basis of a primary CD45/side scatter (SSC) gating procedure. Blast cells were first identified by CD45/SSC gating in 74 cases of acute myeloid leukemia...
While flow cytometry is usually used to identify cell types using phenotypic markers on the cell surface, it can also measure intracellular signaling.
流式细胞术Flow Cytometry,FCM作者zuzh:笛风共八十一页1流式细胞仪Flow Cytometer:是集光电子物理,光电测量,计算机,细胞荧光化学,单抗技术为一体的高科技细胞分析仪。2流式细胞术Flow Cytometry:
Here, we outline a protocol to detect simultaneous expression of several putative LSC markers (CD34, CD38, CD45RA, CD90) on primary blast cells of human AML by multiparametric flow cytometry. Furthermore, we show how to quantify three progenitor populations and a putative LSC population with ...
•Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma[1]•Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer[2]•Frontiers | Flow cytometry quantification of tumor-infiltrating lymphocytes to predict the...
(15;17); APL; immunobead; flow cytometry INTRODUCTION The presence of t(15;17) with the PML–RARA fusion gene is considered to be the hallmark of acute promyelocytic leukemia (APL), also known as acute myeloid leukemia (AML) type M3.1 Initially t(15;17) was assumed to be present in ...
flow cytometry and the other methods, such as genomics, proteomics, immunoprecipitation, fluorescence in situ hybridization (FISH), and epigenetics.The specific clinical relevance of our work is providing reference significance for dynamic monitoring and prognostic observation of acute myeloid leukemia with...