The median (range) time for reporting of PB/BM examination, molecular genetic studies and flow cytometry for CD26+ CML LSCs was 5 (3-11 days), 4 (3-6 days) and 1 (0-1 day) respectively. CD26+ LSCs were identified in all patients with a confirmed diagnosis of CML (Median=0.07%,...
In many hematological malignancies, flow cytometry is the method of choice for diagnosis and monitoring the course of disease. However, to date, no method has been available to identify and quantify BCR-ABL1-positive cells using flow cytometry, at least to our knowledge. The present approach com...
Four-color flow cytometry identifies virtually all cytogenetically abnormal bone marrow samples in the workup of non-CML myeloproliferative disorders. Am J Clin Pathol. 2003;120:854–65.PubMedCrossRef Li S, Eshleman JR, Borowitz MJ. Lack of surface immunoglobulin light chain expression by flow ...
The leukemia cells are lysed and incubated with beads and the detection antibody, then washed and evaluated by flow cytometry for PE positivity of the immunobeads. Using the same criteria, the anti-RARA antibody was raised against sequences downstream of the RARA break point region (Figure 2b)...
Flow cytometry CVs were generally high but improved subsequent to standardisation of sampling and the gating strategy. A similar pattern in the variability of results was observed for platelet concentrates. Sign tests using all samples (... J Seghatchian - 《Transfusion & Apheresis Science》 被引...
PV and ET on one hand and CML on the other hand. The distinction between ET and PV, however, has to be made on other grounds. 展开 关键词: Key words: megakaryocytes myeloproliferative disorders ploidy flow cytometry DOI: 10.1111/j.1600-0609.1996.tb00717.x 被引量: 14 ...
We studied the potential of multidimensional flow cytometry to evaluate the frequency and maturation/activation status of dendritic cells in minimally mani... CA Savary,ML Grazziutti,B Melichar,... - 《Cancer Immunology Immunotherapy》 被引量: 104发表: 1997年 Induction of CML28-specific cytotoxic...
Immunophenotypic studies using immunofluorescent flow cytometry were performed on the blast cells of 36 patients with acute leukaemia using a panel of eight monoclonal antibodies. Six patients had blasts which co-expressed markers for lymphoid and myeloid differentiation, and which were therefore defined...
flowcytometryUK 2021 takes virtual format 来自 国家科技图书文献中心 喜欢 0 阅读量: 16 作者: G Astles 摘要: The flowcytometryUK 2021 Meeting, formerly One Day flowcytometryUK, will take place virtually and will highlight the diverse areas in which cytometry is a vital resource. There will ...
While flow cytometry for monocyte subset analysis has been heralded as an important diagnostic tool for CMML, there still remain important issues, especially related to false positives and negatives. In this real world study, we assessed monocyte subsets, with cut off values of >94% for MO1 fra...