多参数流式细胞仪-MRD (MFC-MRD)在 AML 中的主要优点是适用性高(>90%患者均可检测)、报告时间短、灵敏度相对较高,以及可以区分活细胞和死细胞,使 MFC-MRD 成为针对实时治疗临床决策制定的理想方法。基于 MFC 的 MRD 评估的灵敏度为 10-3 ...
而多参数流式细胞术 (MFC) 已成为 AML MRD 评估的广泛使用方法。然而,MRD在临床决策和药物开发中的重要性日益增加,故需加强监管审查。欧洲体外诊断法规 (IVDR) 和美国食品药品监督管理局 (FDA) 现在都要求在用于临床试验或诊断之前对 MRD 检测进...
而多参数流式细胞术 (MFC) 已成为 AML MRD 评估的广泛使用方法。然而,MRD在临床决策和药物开发中的重要性日益增加,故需加强监管审查。欧洲体外诊断法规 (IVDR) 和美国食品药品监督管理局 (FDA) 现在都要求在用于临床试验或诊断之前对 MRD 检测进行严格的验证。 Tettero JM等人2023年这篇发表在Cytometry B Clin C...
9名急性髓细胞性白血病患者在随访期间复发,大多数患者在复发前FC-MRD呈阳性。 这项研究加深了我们对FC-MRD和Mol-MRD在指导成人急性白血病治疗中的互补作用的理解。 参考文献:van der Linde R, Gatt PN, Smith S, et al. Measurable Residual Disease (MRD) by Flow Cytometry in Adult B-Acute Lymphoblastic L...
In acute myeloid leukemia (AML), assessment of minimal residual disease (MRD) by flow cytometry (flow MRD) after induction and consolidation therapy has been shown to provide independent prognostic information. However, data on the value of earlier flow MRD assessment are lacking. Therefore, the ...
本研究针对急性髓系白血病(AML)异基因造血干细胞移植(allo-HSCT)后微小残留病(MRD)监测的时机与方法学难题,哈尔滨医科大学附属第一医院团队通过多中心队列研究,整合流式细胞术(MFC)、WT1基因表达及二代测序(NGS)技术,证实移植前1个月MRD联合检测可显著提升疾病进展预测灵敏度至100%,并首次明确NGS在移植后6个月监测...
For example, flow cytometry may not be as effective in this patient population, while TP53 MRD detected through deep sequencing—whether by duplex or error-corrected sequencing—may offer better predictive value. Another important takeaway is that MRD monitoring...
a, Flow cytometric measurable residual disease (MRD) in bone marrow aspirates using the different-from-normal (DfN) method. b, Copy number of CAR-123 per microgram of genomic DNA as measured by qPCR at indicated time points for each patient. c, Flow cytometry quantification of surface CD123...
We’re running out of time on this, and we can’t do it justice, but what about using these assays, next-generation sequencing and flow cytometry, for looking at minimal residual disease [MRD]? Are we there yet? Can we use these in our clinical practice? Adam Bagg, MD: I’ve got ...
“In the past, MRD measurements were primarily performed by flow cytometry or PCR (polymerase chain reaction), but with the introduction of precision analysis technologies, including NGS, it is now possible to detect even the smallest residual cells, making it an important tool for early detection...