细胞培养、冻存、复苏试剂准备 生长培养基:DMEM+10% FBS+1% P.S+4 μg/mLBlasticidin+400 μg/mLG418+0.75 μg/mLPuromycin+125 μg/mLHygromycin 细胞冻存液:90% FBS+10% DMSO Assay Buffer:DMEM+1% FBS+1% P.S 数据展示
Cell viability and focus formation assay Cell viability was examined using Cell Counting Kit-8 (CCK-8) according to the manufacturer’s instructions, as previously reported20. The viability of the control group was deemed 100% survival. For the focus formation experiment, LNCaP cells were transfect...
公司,基于自有的国内最大报告基因平台、慢病毒平台以及最迅速的药研网资讯平台,目前已构建出500余株现货单克隆细胞系产品,覆盖ROR1/DLL3/SLC39A6/TROP2/BCMA/BDCA/TREM2……200余新药热门靶点,其中有90余靶点开发有对应luciferase reporter方法,更有国内独家...
For leptin, the sensitivity limit of the assay was 0.08 ng ml−1 (Millipore, EZRL-83K, RRID: AB_2307316), and, for FGF21, the sensitivity limit was 3.81 pg ml−1 (R and D Systems, MF2100, RRID: AB_2783730). FACS sorting The tuberal region of the hypothalamus from ...
TheiLite® FGF21 Assay Ready Cells are a genetically engineered reporter gene cell line responsive to FGF21 by specific and proportional expression of Firefly Luciferase. The receptor chain FGFR1c is overexpressed on the surface together with the co-factor β-Klotho which has been genetically opti...
Circulating FGF21 levels in 45 breast cancer women (median age 59, range 32–88 years) and 51 age-matched healthy controls were evaluated using a quantitative ELISA assay. Patients’ samples were obtained before surgery ahead of any previous therapy. Breast cancer patients showed significantly ...
Cell counting kit-8 (CCK-8) assay Cellular viability was subjected to CCK-8 (Beyotime Institute of Biotechnology) assay. After the cells were treated, the CCK-8 solution was added to the culture medium (CCK-8 diluted 1:10 in medium), which was then incubated in the dark for 4 h at...
Development of an iLite™ Reporter Gene Cell Line for the Quantification of the Potency of Analogues of Human FGF21We have shown that normalization of drug-induced reporter gene activity, relative to the expression of an internalstandardizationgeneunderthecontro...
200余新药热门靶点,其中有90余靶点开发有对应luciferase reporter方法,更有国内独家/首家CCR8, BDCA, TREM2和GAPR等luciferase的assay平台。 GLP1R/GCGR/GIPR/FGF21细胞系产品列表 部分结果:GM-C09150:H_GLP1R Reporter CHO-K1 Cell Line 使用GLP-1(7-37)药物激活验证结果 GM-C09151:H_GCGR Reporter CHO-K1...
基于自有的国内最大报告基因平台、慢病毒平台以及最迅速的药研网资讯平台,目前已构建出500余株现货单克隆细胞系产品,覆盖ROR1/DLL3/SLC39A6/TROP2/BCMA/BDCA/TREM2……200余新药热门靶点,其中有90余靶点开发有对应luciferase reporter方法,更有国内独家/首家CCR8, BDCA, TREM2和GAPR等luciferase的assay平台。