Antibody 克隆号 OTI1H4 抗原 Full length human recombinant protein of FGF21 produced in HEK293 cell 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 1 mg/mL 纯化类型 Affinity Chromatography 保存液 PBS, pH 7.3, with 1% BSA, 50% glycerol 内含物 0.02% sodium azide 保存...
FGF21 Antibody (PA5-79254) in WB Western blot analysis of FGF21 in Lane 1: A549 whole cell lysate, Lane 2: A431 whole cell lysate, Lane 3: HEPA whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking ...
For fluorescence acquisition, the cells were stained with FITC anti-mouse CD11b Antibody (BioLegend) and PerCp-CyTM5.5 anti-mouse CD45 Antibody (BD Pharmingen). Flow cytometry (FACS Aria II SORP, BD Biosciences, USA) was employed for fluorescence acquisition, and the samples were gated based...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
Chromatin samples were immunoprecipitated with an anti-ATF2 antibody (20F1), an anti-cAMP response element binding protein (CREB) antibody (48H2) (Cell Signaling), or unrelated immunoglobulin (Santa Cruz Biotechnology). Input DNA and immunoprecipitated DNA were analyzed by quantitative PCR using ...
Cell metabolism 16, 387–393, doi:10.1016/j.cmet.2012.08.002 (2012). 9. Foltz, I. N. et al. Treating diabetes and obesity with an FGF21-mimetic antibody activating the betaKlotho/FGFR1c receptor complex. Science translational medicine 4, 162ra153, doi:10.1126/scitranslmed.3004690 (2012)...
be tightly regulated since defective autophagy has been implicated in many diseases, including cancer, neurodegenerative disease, and metabolic disorders like obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD), while excessive autophagy is also harmful because it promotes cell death4,5,6...
Previously, recombinant antibody proteins that activate the FGFR1/βKlotho complex were proposed to act as an FGF21-mimetic; however, in vivo action of these engineered proteins has not been well studied. Methods We investigated the mechanism by which anti-FGFR1/βKlotho bispecific antibody (bFKB1...
The antibody is a mouse monoclonal antibody raised against FGF21. It has been selected for its ability to recognize FGF21 in immunohistochemical staining and western blotting. USAGEWestern blotting: 0.5-2µg/mL;Immunohistochemistry: 5-20µg/mL;Immunocytochemistry: 5-20µg/mL;Optimal working ...
A primary antibody (anti-pFGFR Tyr653/654 (New England Biolabs, Frankfurt, Germany) or anti-pERK Phospho-p44/42 MAP Kinase Thr202/Tyr204 (Cell Signaling)) was added and incubated overnight at 4° C. After incubation of the primary antibody, cells were washed with PBS plus 0.1% Tween20...