Normal growth characterised as trajectories within the interquartile range (25th–75th) on the GROW chart had a protective effect for EmCS (RR 0.64, CI 0.57–0.72) and Comp (0.67, 0.53–0.86), and no significant association with perinatal death (1.06, 0.51–2.18). Pregnancies with growth traj...
22. The assay sensitivity can be improved by sampling shorter cfDNA fragments from the maternal plasma when a higher percentage of fetal cfDNA molecules
The percentage of a maternal cell-free DNA (cfDNA) sample that is fetal-derived (the fetal fraction; FF) is a key driver of the sensitivity and specificity of noninvasive prenatal screening (NIPS). On certain NIPS platforms, >20% of women with high body mass index (and >5% overall) rec...
However, the ability to detect aneuploidy with cfDNA analysis is dependent upon assay precision and fetal DNA percentage in the sample rather than the prevalence of the disease in the study population. Most studies were also classified as being at high risk of bias in relation to flow and ...
Current relevance of non-invasive prenatal study of cell-free fetal DNA in the mother’s blood and prospects for its application in mass screening of pregnant women in the Russian Federation
It turns out, that almost all of the cffDNA found in the maternal blood stream actually comes from the DNA of the cells that form the placenta or perform the anchoring function, not the cells that form the fetus. Therefore, it’s not entirely accurate to use “cell freefetalDNA” when ...
ART, assisted reproduction technology; BMI, body mass index; cfDNA, cell-free DNA; IQR, interquartile range (25th − 75th per- centile); NC, not comparable; PPV, positive predictive value. The mean FF was 10.6% (± 2.9). No correlation was found between FF and gestational age at ...
The size range of fragments had a major impact on the efficiency of enrichment of fetal DNA. PCR was used to amplify cfDNA, and e-gel electrophoresis was used to screen out the size of different cfDNA fragments. We found that the optimal size of DNA fragments was 125-135 bp, and ...
miRNA;NIPT;low cell-free DNA (cfDNA) fetal fraction 1. Introduction Non-invasive prenatal testing (NIPT) is a sophisticated method used worldwide for testing chromosomal abnormalities in the fetus. Currently, NIPT is considered to be a valuable test for pregnant women to provide a cost-effective...