cffDNAPrenatal screeningAneuploidyPrenatal diagnosisWhole-genome sequencingChromosomal abnormalitiesThe prenatal test of cell-free fetal DNA (cffDNA) is also known as noninvasive prenatal testing (NIPT) with high sensitivity and specificity. This study is to evaluate the performance of NIPT and its ...
DNAinmaternalbloodnon-invasiveprenatalCell free fetal DNA (cff-DNA) in maternal blood has given rise to the possibility of new non-invasive approaches in prenatal genetic diagnoses. In contrast to the established invasive techniques chorionic villi sampling and amniocentesis, both associated with ...
The cell-free fetal DNA (cffDNA) test for fetal sex determination is performed by establishing whether the Y chromosome is present or absent in the maternal plasma by targeting Y chromosome-specific sequences using real-time quantitative polymerase chain reaction (RT-qPCR). Fetal rhesus D (RHD...
Cell-free fetal DNA (cffDNA) in maternal peripheral blood, discovered by Lo et al.1, makes it possible to infer the fetal inheritance in a noninvasive way and led to the development of noninvasive prenatal applications, such as fetal sex determination for sex-linked disorders2, detection of...
To determine the association between cell-free DNA fetal fraction (cffDNA) and various prenatal characters to better guide the clinical application of noninvasive prenatal screening (NIPS), a retrospective cohort study of 27,793 women with singleton pregnancies was conducted. Results indicated that no...
Cell-free fetal DNA and adverse outcome in low risk pregnancies. 1.6% preterm birth Conclusion: Cff DNA in maternal plasma of the second trimester was not found to be a marker for an adverse pregnancy outcome in low... W Stein,S Müller,K Gutensohn,... - 《European Journal of Obstetrics...
Isolationandanalysisofcell-freefetalDNAfrom464646 系统标签: fetaldnaisolationcffdnanipdmaternal ©FUNPEC-RP.funpecrp.brGeneticsandMolecularResearch14(4):18078-18089(2015) Isolationandanalysisofcell-freefetalDNA frommaternalperipheralbloodinChinese women W.C.Yang 1 *,L.Zhu 1 *,Y.M.Qiu 1,2 *,B...
The aim of this study was to determine whether the increased serum cell-free fetal DNA (cffDNA) level of gravidas developed into early-onset preeclampsia (EOPE) subsequently in the early second trimesters is related to prenatal screening markers. Serum was collected from 1011 gravidas. The lev...
Figure 4 Fetal sex determination using PCR of cell-free fetal DNA (cff-DNA) in maternal plasma. (a) Case 1; (b) Case 2; (c) Case 3. Lanes labeled 9, 10, 11 and 12 indicate cff-DNA at the respective week of gestation; FF and MF indicate cff-DNA from a woman pregnant with a...
The fraction of circulating cell-free fetal (cff) DNA in maternal plasma is a critical parameter for aneuploidy screening with non-invasive prenatal testing, especially for those samples located in equivocal zones. We developed an approach to quantify cff DNA fractions directly with sequencing data,...