The discovery of cell-free fetal DNA circulating in the maternal blood has great potential for the development of NIPT methodologies. Such strategies have been successfully applied for the determination of the fetal rhesus status and inherited monogenic disease, but the field of fetal aneuploidy ...
The fetal fraction, or percentage of total cell-free DNA (cfDNA) in a sample derived from the fetus, can impact NIPT results and profitability.
Cell-free fetal DNA in maternal blood: new possibilities in prenatal diagnostics1) Markus Stumm1, 2, 4 / Rolf-Dieter Wegner1, 2 / Wera Hofmann3 1Center for Prenatal Diagnostics and Human Genetics Kudamm 199, Berlin, Germany 2BG Berlin-Genetics GmbH, Berlin, Germany 3LifeCodexx AG, ...
Fetal DNA is present in maternal capillary blood allowing for noninvasive fetal sex determination. Exogenous male DNA may be present, and detectable, on the fingertips of pregnant women. The elimination of the exogenous male DNA is critical for a reliable fetal sex determination using maternal ...
Cell-free foetal DNA recently hit the international headlines by facilitating the non-invasive prenatal testing (NIPT) of foetal chromosomal anomalies directly from maternal blood samples. Being largely of placental origin, cell-free foetal DNA may also, however, provide insight into underlying ...
Maron JL,Johnson KL,Bianchi DW.Cell-free fetal DNA plasmaextraction and real-time polymerase chain reaction quantification. Methods in Molecular Medicine . 2007Maron J L,Johnson K L,Bianchi D W.Cell-free fetal DNA plasma extraction and real-time polymerase chain reaction quantification[J].Methods...
there have been numerous attempts to use fetal nucleated cells that have entered into maternal blood for prenatal testing. However, the rarity of such cells has impeded this approach from becoming a clinical reality. Because cell-free tumor DNA has been detected in the plasma of patients with ...
We evaluated the feasibility of cffDNA extraction from the maternal blood samples regarding the threshold concentrations required for fetal sexing in pregnant cattle by PCR. In four trials, we 1) compared the extraction efficiency of seven methods using freshly harvested plasma/blood of cows carrying...
aeinem Arbeitspensum von 100% 工作负担100%[translate] anotificación 通知[translate] aFetal cells and cell-free fetal DNA in maternal blood: new insights into pre-eclampsia 胎儿细胞和无细胞胎儿脱氧核糖核酸在母亲血液: 新的洞察到子痫前期里[translate]...
Bischoff FZ, Lewis DE, Simpson JL. Cell-free fetal DNA in maternal blood: kinetics, source and structure.Hum Reprod Update. 2005;11(1):59–67. CASPubMedGoogle Scholar Ariga H, Ohto H, Busch MP, et al. Kinetics of fetal cellular and cell-free DNA in the maternal circulation during and...