And ferroptosis-related DEGs (FRDEGs) were screened by venn diagram with ferroptosis-related genes downloaded from FerDb database. FRDEGs was enriched and analyzed by GO and KEGG, and hub genes related to ferroptosis were screened by Cytoscape software and database STRING. Additionally, considering...
From these DEGs, we extracted ferroptosis-related DEGs. GSE89632, another microarray dataset, was used to validate the expression of ferroptosis-related genes. A protein–protein interaction (PPI) network of ferroptosis-related genes was then constructed. The target genes were also subjected to Gene...
GPX4 is the only enzyme that directly reduces hydrogen peroxide from biofilm lipids [34]. Suppressing the system XC−/GSH/GPX4 axis results in the accumulation of LPO, thereby leading to ferroptosis; for example, system XC−activity is directly inhibited...
Then, we constructed a prognostic multigene signature with ferroptosis-related differentially expressed genes (DEGs) in the TCGA cohort and validated it in the ICGC cohort. Finally, we further performed functional enrichment analysis to explore the underlying mechanisms....
First, the functions of FRGs that were differentially expressed and were upregulated and downreg- ulated (DEGs) were examined. With Gene Ontology (GO), DEG-related biological pathways were analyzed. Biological processes (BP), molecular functions (MF), and cellular com- ponents (CC) controlled...
We used ferroptosis-related genes as the basis, and identify the differential expression genes (DEGs) between cancer and paracancer. The univariate Cox regression analysis were used to screen the prognostic-related genes. We divided the patients into training and validation sets. Then, we screened ...
Our focus extended specifically to ferroptosis-related DEGs (FRDEGs), cuproptosis-related DEGs (CRDEGs), anoikis-related DEGs (ARDEGs), autophagy-related DEGs (AURDEGs), and pyroptosis-related DEGs (PRDEGs). Further scrutiny involved ... T Dong,X Li,W Yu,... - 《Scientific Reports》 被...
PPI, Protein-Protein Interaction; OFRGs, oxidative stress and ferroptosis-related genes; PCA, principal component analysis; t-SNE, t-distributed stochastic neighbor embedding; K-M, Kaplan-Meier; ssGSEA, single sample gene set enrichment analysis; GSVA, gene set variation analysis; DEGs, ...
The analysis included samples from four patients, revealing a total of 129 differentially expressed genes (DEGs), consisting of 85 upregulated genes and 44 downregulated genes (|log2FC| ≥ 0.5; padj≤0.05) (Fig. 7A-B, Supplementary table 3). We performed functional enrichment analysis using ...
a Venn diagram to identify ferroptosis-related DEGs between tumor and adjacent normal tissue that were correlated with OS. b Forest plots showing the results of univariate Cox regression analysis between the expression of 10 candidate FRGs and OS. c Heat map showing the expression of 10 candidate...