The term protein 'family' has been used to describe groups of related enzymes that share structural and mechanistic properties. A beautiful example for such an ensemble is the FAD-dependent NAD(P)H-(disulphide)-oxidoreductases. Crystal structures for several family members have recently been ...
quinone oxidoreductase (NDH-2) family and is divided into three domains: the 6-OH-FAD-binding domain (residues 10–111 and 246–327), the NAD(P)H-binding domain (residues 112–245), and the C-terminal membrane-anchoring domain (CTD, residues 328–373) (Supplementary Fig.S1a–c). The...
The FAD-dependent oxidoreductase from Chaetomium thermophilum ( Ct FDO) is a novel thermostable glycoprotein from the glucose–methanol–choline (GMC) oxidoreductase superfamily. However, Ct FDO shows no activity toward the typical substrates of the family and high-throughput screening with around 1000 ...
amino acid sequences namely, the FAD binding motif (Gly-x-Gly-x-x-Gly), the amino acids around the substrate binding pocket, the and expected active site amino acids (His), are concerved, and these are the signature features of the glucose methanol choline (GMC) oxidoreductase family. How...
The associated reductase component HadX has similarity to other NADH:flavin oxidoreductases like TcpX, TftC, and HpaC, but is so far not characterized in detail [100]. 2.1.4. Biotechnological Relevance of Group D Flavoprotein Monooxygenases Hydroxylated aromatic compounds are of interest for ...
FOXRED1 FAD-dependent oxidoreductase domain containing 1 [Homo sapiens (human)] This gene encodes a protein that contains a FAD-dependent oxidoreductase domain. The encoded protein is localized to the mitochondria and may function as a... entrezgene - 《Observational Study of Gene Disease Associati...
Monolignol oxidoreductases are members of the berberine bridge enzyme鈥搇ike (BBE-like) protein family (pfam 08031) that oxidize monolignols to the corresponding aldehydes. They are FAD-dependent enzymes that exhibit the para-cresolmethylhydroxylase-topology, also known as vanillyl oxidase-topology. ...
A phylogenetic analysis revealed six more novel-type TrxR groups and suggested that the broader flavin-containing disulfide oxidoreductase family is more diverse than previously considered. The unprecedented structural similarities between an F 420 -dependent enzyme (DFTR) and an NADPH-dependent enzyme (...
2.6. Characterization of AA_3 Glucose Oxidoreductases 2.6.1. Enzyme Activity Assays and Protein Measurement The concentration of protein was determined by Bradford’s assay using the Bio-Rad Protein assay kit containing BSA as standard, measured in a DU 800 UV/Visible Spectrophotometer (Beckman Coul...