We have examined the biogenesis of the F 1F 0 ATP synthase subunit c, a small double spanning IMP, using complementary in vivo and in vitro approaches. The data suggest that F 0c is targeted by the SRP to the membrane, where it inserts at YidC in a Sec-independent mechanism. F 0c...
H+-ATPase activity of Escherichia coli F1F0 is blocked after reaction of dicyclohexylcarbodiimide with a single proteolipid (subunit c) of the F0 complex. Dicyclohexylcarbodiimide (DCCD) specifically inhibits the F1F0-H+-ATP synthase complex of Escherichia coli by covalently modifying a proteolipid su...
Interestingly, all known proteins that strictly require YidC for membrane insertion, the major coat proteins of the bacteriophages Pf3 and M13 (21), F0c of the F1F0 ATP synthase, and subunit a of the cytochrome bo3 oxidase subunit, assemble into large oligomeric structures, which may point ...
immunofluorescence analysis showed that the α2δ-1 subunit and the F-ATPase β-subunit colocalize on the cell surface rather than in intracellular mitochondria (Fig.2c). To further
establishedoftheindensity・induced(confluency—induced)HaCaTdifferentiation,theexpressionofthe0subunitofF1FO-ATPase(synthase)(ATP5B)weretestedbyWesternBlotandRT-PCR,respectively3Theimpactofsmall—moleculeinhibitors(oligomycinandpiceatann01)OtltheintracellularandextracellularATPweredetectedbyATPquantitative...
(passive or active), depending on the transmembrane proton gradient9,10. F1F0-ATP synthase is composed of 16–18 subunits, including α, β, γ, δ, ε and a, b, c, that respectively constitute two major sectors, the F1 and F011. The β subunit (ATP5B) contains the ATP and ADP ...
Taken together, the dimer-specific subunit Su e, and the inhibitor protein Inh1 and its accessory proteins do not display an interdependence on each other for their stable expression. These observations are consistent with those presented previously, where the formation of the dimeric ATP synthase,...
YidC is a member of the Oxa1 family of proteins that facilitates the membrane insertion of a subset of inner membrane proteins in Escherichia coli. YidC acts as an insertase for membrane insertion of subunit c of the F1F0 ATP synthase (F0c), but the requirements for substrate recognition...
Defining the Domain of Binding of F1 Subunit ε with the Polar Loop of F0 Subunit c in theEscherichia coli ATP Synthase We have previously shown that the E31C-substituted ε subunit of Fcan be cross-linked by disulfide bond formation to the Q42C-substitutedsubunit of Fin the... J Hermol...
Cui, Y., Zhang, Y., Yue, J.et al.Direct observation of the clockwise light-driven rotation of F0F1-ATP synthase complex.Chin. Sci. Bull.49, 1342–1347 (2004). https://doi.org/10.1360/04wc0009 Download citation Received07 January 2004 ...