A threefold increase in Vmax (nmol/min/mg) (6.35 ± 0.17 vs. 2.08 ± 0.06) and an eightfold increase in Km (µM) (7.5 ± 0.7 vs. 0.85 ± 0.5) by F1F0 ATP synthase for iron uptake were observed in iron-overloaded mitochondria. Mitochondrial ATP synthase prepared from iron-overloaded...
F1-F0ATP合成酶beta亚基 1. Objective:To prepare the monoclonal antibodies against human F1-F0 ATP synthase beta subunit (hATP5B) and to study their specificities as well as anti-tumor activity. 目的:制备鼠抗人F1-F0ATP合成酶beta亚基(hATP5B)单抗,并对其特异性和抗肿瘤活性进行研究。 2) prolyl...
As a transmembrane enzyme, ATP synthase plays an important role in energy metabolism of organ tissues, as well as in tumors. In this study we generated a monoclonal antibody, 6G11, to the catalytic subunit of F1-F0 ATP synthase (ATP5B). The SDS-PAGE result demonstrated that the hybridoma ...
The F1F0-ATP synthase, an enzyme complex, is mainly located on the mitochondrial inner membrane or sometimes cytomembrane to generate or hydrolyze ATP, play a role in cell proliferation. This study focused on the role of F1F0-ATP synthase in keratinocyte differentiation, and its relationship with...
As a multi-subunit protein localized in the mitochondria of eukaryotic cells, the F 0F 1-ATP synthase α belongs to the family of stress proteins HSP60. Currently, mounting evidences indicate F 0F 1-ATP synthase α may play a role in neurodegenerative diseases, including Alzheimer's disease ...
The yeast F1F0-ATP synthase forms dimeric complexes in the mitochondrial inner membrane and in a manner that is supported by the F0-sector subunits, Su e and Su g. Furthermore, it has recently been demonstrated that the binding of the F1F0-ATPase natural inhibitor protein to purified bovine...
Plant chloroplasts, in contrast, use a different mechanism to inhibit the hydrolysis activity of ATP synthase. Here, the γ subunit encoded by atpC responds to redox signals, thereby preventing the back reaction of ATP synthase when the photosynthetic proton gradient ceases, particularly during the ...
In all Acholeplasma, Mycoplasma and Spiroplasma species tested, a protein capable of reacting with antibodies prepared against the beta subunit of the proton-ATPase complex from yeast, chloroplasts and Escherichia coli was detected. The reactive protein of M. gallisepticum was found to be catalytically...
Neutrophils form the first line of innate host defense against invading microorganisms. We previously showed that F0F1 ATP synthase (F-ATPase), which is widely known as mitochondrial respiratory chain complex V, is expressed in the plasma membrane of hum
F1 and F0 connections in the bovine mitochondrial ATP synthase: the role of the of alpha subunit N-terminus, oligomycin-sensitivity conferring protein (OCS... Xu T,Gaballo A,Zanotti F,et al.F1 and F0 connections in the bovine mitochondrial ATP synthase:the role of the alpha subunit of N-...