A threefold increase in Vmax (nmol/min/mg) (6.35 ± 0.17 vs. 2.08 ± 0.06) and an eightfold increase in Km (µM) (7.5 ± 0.7 vs. 0.85 ± 0.5) by F1F0 ATP synthase for iron uptake were observed in iron-overloaded mitochondria. Mitochondrial ATP synthase prepared from iron-overloaded...
1. Objective:To prepare the monoclonal antibodies against human F1-F0 ATP synthase beta subunit (hATP5B) and to study their specificities as well as anti-tumor activity. 目的:制备鼠抗人F1-F0ATP合成酶beta亚基(hATP5B)单抗,并对其特异性和抗肿瘤活性进行研究。
The F1F0-ATP synthase was once thought to be restricted on mitochondrial inner membrane in eukaryotic cells, but recent studies have shown that it is also located on the cytoplasmic membrane of keratinocytes14, vascular endothelial cells15, adipocytes16, NSCLC cells17,18, and osteosarcoma cells19....
Three positive (PR1-3) and one negative (NR1) transcriptional control domain have been tentatively mapped in the promoter of the human F0F1-ATP synthase beta subunit gene (ATPsyn beta) in the context of expression in myogenic cells. Lipofection of promoter-chloramphenicol acetyltransferase fusion ...
Moreover, examination of the ATP synthase genetic locus indicates various gene rearrangements in the different bacterial lineages, ancient duplications of atpI and of the beta subunit of the F-0 subcomplex, as well as more recent stochastic lineage-specific and species-specific duplications of all ...
(D) Probing the elution fractions of PatpT::atpT-3xFLAG, PatpT::atpT and PpetJ::3xFLAG-sfgfp with anti-AtpB serum. As a further control experiment, we tested the enrichment of AtpB (subunit beta of ATP synthase) in an eluate from the pull-down experiment with FLAG-tagged AtpΘ by...
Rotary catalysis in FFATP synthase is powered by proton translocation through the membrane-embedded Fsector. Proton binding and release occurs in the middle of the membrane at Asp-61 on transmembrane helix 2 of subunit . Previously, the reactivity of cysteines substituted into Fsubunit revealed two...
The structure of the [delta ] subunit has been determined by NMR, as has the structure of the [epsiv ] subunit. Cysteine residues have been incorporated at multiple positions in the [alpha ], [beta ], [gamma ], [delta ], [epsiv ] and c subunits by site-directed mutagenesis. These ...
subunit 1 (COX-1) and NADH dehydrogenase. In vivo studies also suggested that accumulation of this deletion caused a sharp decline in the mitochondrial mRNA transcript levels [15]. These, thus, have a drastic effect on the oxidative phospho...
Site-directed cassette mutagenesis was used to generate a series of amino acid substitutions in the a subunit of the Escherichia coli F1F0-ATP synthase. The following substitutions for Asn-192 were analyzed and shown to inhibit partially the ATP-dependent proton translocation without disrupting F1-F0...