Driessen, Sub- unit a of the F1F0 ATP Synthase Requires YidC and SecYEG for Membrane Insertion, J. Mol. Biol. 390 (2009) 893-901.Kol S, Majczak W, Heerlien R, van der Berg JP, Nouwen N, Driessen AJ. 2009. Subunit a of the F1F0 ATP synthase requires YidC and SecYEG for...
Subunit-Subunit-Interactions in F0F1 as Revealed by Ligand Binding and Intrinsic FluorescenceFor a better understanding of the molecular mechanism of the H + -ATP-synthase, a prerequisite is to know more about the correlation between structural and functional aspects. One possibility to study this ...
F1-F0ATP合成酶beta亚基 1. Objective:To prepare the monoclonal antibodies against human F1-F0 ATP synthase beta subunit (hATP5B) and to study their specificities as well as anti-tumor activity. 目的:制备鼠抗人F1-F0ATP合成酶beta亚基(hATP5B)单抗,并对其特异性和抗肿瘤活性进行研究。 2) prolyl...
F0F1 ATP synthase cross‐linkingmutationphotolabelingsubunit ɛCys residues were directed into positions 17, 28, 41 and 85 of a Cys6 → Ser mutant of subunit of spinach chloroplast F0F1 ATP synthase. Wild-type and engineered were expressed in Escherichia coli, purified in the presence of ...
As a transmembrane enzyme, ATP synthase plays an important role in energy metabolism of organ tissues, as well as in tumors. In this study we generated a monoclonal antibody, 6G11, to the catalytic subunit of F1-F0 ATP synthase (ATP5B). The SDS-PAGE result demonstrated that the hybridoma ...
The addition to F0 liposomes of γ subunit, but not that of α and β subunits, caused per se inhibition of H+ conduction. It is concluded that F0I-PVP and γ subunits are directly involved in the gate of the F0F1 H+-ATP synthase. Data are also presented indicating contribution to ...
As a multi-subunit protein localized in the mitochondria of eukaryotic cells, the F 0F 1-ATP synthase α belongs to the family of stress proteins HSP60. Currently, mounting evidences indicate F 0F 1-ATP synthase α may play a role in neurodegenerative diseases, including Alzheimer's disease ...
(passive or active), depending on the transmembrane proton gradient9,10. F1F0-ATP synthase is composed of 16–18 subunits, including α, β, γ, δ, ε and a, b, c, that respectively constitute two major sectors, the F1 and F011. The β subunit (ATP5B) contains the ATP and ADP ...
Moreover, examination of the ATP synthase genetic locus indicates various gene rearrangements in the different bacterial lineages, ancient duplications of atpI and of the beta subunit of the F-0 subcomplex, as well as more recent stochastic lineage-specific and species-specific duplications of all ...
Plant chloroplasts, in contrast, use a different mechanism to inhibit the hydrolysis activity of ATP synthase. Here, the γ subunit encoded by atpC responds to redox signals, thereby preventing the back reaction of ATP synthase when the photosynthetic proton gradient ceases, particularly during the ...