Using pharmacological inhibitors that perturb individual aspects of actomyosin dynamics without disassembling the network, we demonstrate that F-actin polymerization is the primary driver of actin retrograde flow, whereas myosin IIA promotes long-term integrity of the IS. Disruption of F-actin retrograde...
Activation of the Arp2/3 complex by N-WASP is required for actin polymerization and contraction in smooth muscle. Am. J. Physiol. 288, C1145-C1160. Chen et al., 2004. Protective effect of phosphatidylinositol 4,5-bisphosphate against cortical filamentous actin loss and insulin resistance ...
Recently we demonstrated the existence of a phosphatidylinositol 3-kinase (PI3K)–independent F-actin polymerization during neutrophil pseudopod extension. Here we examine the use of the PI3K-dependent and PI3K-independent pathways of activation by the N-formyl peptide receptor and the chemokine receptor...
名称及描述cat#规格说明 Tubulin polymerization assay using >99% pure tubulin, OD based - PorcineBK006P24-30 assays比色法 Tubulin polymerization assay using >99% pure tubulin, fluorescence basedBK011P96 assays荧光法 二、Cytoskeleton活细胞微丝蛋白F-actin的染色&肌动蛋白聚合分析 #F-actin活细胞染色: 关...
Fig. 4. Local sites with low MPA density direct local membrane protrusions by Rac-mediated actin polymerization and osmotic pressure. Fig. S8 Locally low MPA density directs osmotically driven membrane protrusions and inhibits existing ...
Zhang et al., 2005. Activation of the Arp2/3 complex by N-WASP is required for actin polymerization and contraction in smooth muscle. Am. J. Physiol. 288, C1145-C1160. Chen et al., 2004. Protective effect of phosphatidylinositol 4,5-bisphosphate against cortical filamentous actin loss and...
Since alteration in F-actin level influences on the formation of vacuoles/vesicles during exocytosis/endocytosis in astrocytes, we investigated whether F-actin polymerization involves clasmatodendrosis in the rat hippocampus following status epilepticus (SE). In the present study, vacuoles in clasmato...
(1.广东省农业科学院动物卫生研究所，广东省兽医公共卫生公共实验室，广东省畜禽疫病防治研究重点实验室，广州510640；2.华南农业大学兽医学院，广州510642)摘 要：病原入侵宿主细胞，由宿主细胞肌动蛋白(F -a c t i n )聚集引起的细胞骨架重排起着极其重要的作用，本文主要就宿主细胞整合素介导的宿主细胞F -a c...
Using both genetic and pharmacological approaches, we show that excess F-actin polymerization leads to impaired autophagic activity and the accumulation of dysfunctional mitochondria in the aged brain. Remarkably, we show that treating aged animals with cytoskeletal drugs, to disrupt actin polymerization,...
The Effects of Intracellular F-action Polymerization and Depolymerization on Bel-7402 Hepatocarcinoma Cell Line (林珏龙 ,陈耀文 ,蔡维佳 , 等. 细胞内 F2actin 的聚合与解聚对肝癌细胞 Bel27402 的影响 [J ] . 激光生物学报 ,2005 ,14 (1) : 52255. )... JL Lin,YW Chen,WJ Cai,... - 《Acta...