BSA02-001Actin Polymerization Buffer (10x stock)1 x 2 ml2203.6 BSA04-001ATP (100mM Stock)1 x 1 ml2203.6 CF01-A肌动蛋白解聚因子Cofilin (人源重组)1 x 100 µg7939.6 CF01-C4 x 100 µg25277.0 CS-MH03重酶解肌球蛋白 (HMM Fragment)1 x 100 µg10532.1 ...
The recombinant protein was then eluted in GST elution buffer (50 mM Tris-Cl, pH 8.0; 50 mM NaCl; 10 mM GSH). F-actin cosedimentation assay Actin was purchased from Cytoskeleton, Inc. and polymerized by addition of actin polymerization buffer (5 mM Tris-HCl, pH 8.0; 0.2 ...
Polymerized non-muscle actin was mixed with His-MBP-fusion proteins in actin polymerization buffer (final volume 100 µl). The dialysis buffer was used as balance. After incubation at RT for 1 h, filamentous actin was sedimented by ultracentrifugation (1.5 h, 150,000 g), and both...
8. SDS sample buffer (5 x) 9. DMSO 10. Manual with detailed protocols and extensive troubleshooting guide 保存条件 参考产品说明书,按照要求保存各组分 * 本产品仅适用于科研用途. 常见问题FAQ: G-Actin/F-Actin比值分析试剂盒的实验中,进行到哪一步,可暂停实验? 直到在37°C下以100,000xg旋转1小时后...
Abstract We report measurements of small angle neutron scattering from solutions of rabbit muscle G-actin at 3.00 mg/mL in D 2 O buffer solution, with [ Ca 2+ ]=0.52 mM and with [KCl]=15, 8.9, and 5.4 mM. We observe the onset of the polymerization of G-actin to F-actin as ...
Actin Polymerization Buffer (10x stock)| 基本信息更多信息 中文名称: 中文同义词: 英文名称:Actin Polymerization Buffer (10x stock) 英文同义词:Actin Polymerization Buffer (10x stock) CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: ...
Actin was polymerized by diluting 1:1 in 2× polymerization buffer (100 mm KCl, 20 mm imidazole, pH 7.4, 0.2 mm MgCl2, 1 mm EGTA) or in a solution of 2× polymerization buffer containing cortactin at 10 mg/ml followed by incubation on ice for 5 h. Murine cortactin, splice isoform 1...
Beijing 100094, China;.Expression of Chlamydomonas actin-gfp fusion gene in tobacco suspension cell and polymerization of the actin-gfp protein in vitro[J]... Y Yang - 《科学通报(英文版)》 被引量: 64发表: 2001年 Expression ofChlamydomonas actin-gfp fusion gene in tobacco suspension cell and...
Zhang et al., 2005. Activation of the Arp2/3 complex by N-WASP is required for actin polymerization and contraction in smooth muscle. Am. J. Physiol. 288, C1145-C1160. Chen et al., 2004. Protective effect of phosphatidylinositol 4,5-bisphosphate against cortical filamentous actin loss and...
The actin polymerization reaction was initiated by adding polymerization buffer (25 mM Tris, pH 7.0, 50 mM KCl, 2 mM MgCl2, 0.1 mM ATP) and the mixture was incubated at 23 °C for 5 min. Actin filaments were recovered by ultracentrifugation at 157,000×g for 20 min ...