rheological properties of bloodThe motion of many intracellular pathogens is driven by the polymerization of actin filaments. The propulsive force developed by the polymerization process is thought to arise from the thermal motions of the polymerizing filament tips. Recent experiments suggest that the ...
N.: Polymerization of actin. VI. The polarity of the actin filaments in the acrosomal process and how it might be determined - Tilney, Kallenbach - ... LG Tilney,N Kallenbach - 《Journal of Cell Biology》 被引量: 63发表: 1979年 Acrosomal Reaction ofThyoneSperm II: The Kinetics and Pos...
Since filaments are not present in the periacrosomal region during the accumulation of the actin even though the fixation of these cells is sufficiently good to distinguish actin filaments in thin section, the actin must accumulate in the nonfilamentous state. The membranes in the periacrosomal ...
To distinguish growth at barbed and pointed ends we used image drift correction and maximum intensity projections to reveal points where single N-ethylmaleimide inactivated myosins attach filaments to the glass coverslip. We estimated association rates at high actin concentrations and dissociation rates ...
Nucleators generating new F-actin filaments play important roles in cell activities. Detailed information concerning the events involved in nucleation of actin alonein vitrois fundamental to understanding these processes, but such information has been hard to come by. We addressed the early process of...
Electron microscopy of negatively stained samples indicates that caldesmon induces polymerization of Gactin into filaments. Polymerization takes place in a very low ionic strength solution and is accompanied by an increase of intensity of fluorescence of G-actin labelled with N-(1-pyrenyl)iodoacetamide....
The actin polymer, like the thin filaments of muscle, is known to be polarized as demonstrated by the well known "HMM decoration" technique to give a "herringbone" pattern pointing in one direction. The question "Does polymer formation proceed unidirectionally, or bidirectionally?" was raised and...
These results show that ATP-actin occupies both ends of actin filaments growing in ATP. The bound ATP must be split on internal subunits and the number of terminal subunits with bound ATP probably depends on the rate of growth. 展开 关键词:...
degree of polymerization聚合度 condensation polymerization缩聚反应;缩聚合 anionic polymerization阴离子聚合 free radical polymerization自由游离基(引发)聚合 interfacial polymerization[物]界面聚合 cationic polymerization阳离子催化聚合作用 thermal polymerization热聚合 ...
In order to understand the role of actin microfilaments in the apoptotic process, we followed their evolution during tumor necrosis factor-α (TNF)-induced apoptosis in bovine aortic endothelial (BAE) cells. Using Western blotting analysis and immunofluorescence microscopy, we observed that the actin...