filament. The hydrolysis of ATP is important in determining the critical concentration of free actin at the plus and minus ends of the filament. This leads to simultaneous assembly of new subunits at the plus end and disassembly of subunits from the minus end, a process known as ‘treadmilling...
hydrolysed and phosphate is released (Carlier and Pantaloni, 1988). The resulting ADP-actin filaments are disassembled through the loss of actin monomers from the filament ‘pointed’ (minus) end and by the action of actin-severing proteins (Bamburg et al., 1999). This cycle of actin ...
To address whether actin might be involved in spindle assembly, we first tested in metaphase II oocytes for a co-localization with the γ-tubulin rich minus ends of microtubules, the origin of nucleation and growth initiation. We observed filamentous actin clusters overlapping at the spindle poles...
16,17. The dimers self-polymerize to produce a continuous strand that wraps around the F-actin helix. Locally, individual dimers associate weakly with the thin filament; yet, the full-length Tpm cableas a wholeinteracts strongly with F-actin18,19,20,21. This purported Gestalt-binding behavior...
Structural basis of filament assembly and disassembly mechanisms The assembly of actin filaments from monomers is a two-step process that involves (a) the formation of an energetically unfavorable nucleus of G-actin dimers or trimers, followed by (b) the elongation of the nucleus by the addition...
or as a filament- F-actin. G-actin can spontaneously assemble into F-actin, forming a polarised polymer. Filament polymerization occurs primarily at the barbed-end (or plus-end), whereasdepolymerisationoccurs mainly in pointed-end (or minus-end). Multipleactin binding proteins(ABPs) can facilit...
Each time an intersection was found, the segment was cut at that point, the current length from either the filament start or the last intersection point was recorded, and the process continued from the intersection point until the other end of the filament was reached. Kymographs were produced...
Refers to the movement of cytoplasm driven either by actomyosin contractility or by microtubule-based organelle movement. It is most common in plants and algae, but it also occurs during oogenesis in the fruitfly and during embryogenesis in Caenorhabditis elegans. Microtubule minus-end trackers (–...
Col-0 and thecpk3-2mutant. Thus, CPK3 does not appear to be required for maintenance of cellular G- and F-actin levels and/or the ratio of the two. Based on this, we surmise that CPK3′s primary role, as a function of the cytoskeleton, is the regulation of actin filament dynamics...
Many reports indicate that S100A6 is involved in actin filament organization, however the mechanism of S100A6 action in this process is not fully understood. By screening S100A6 binding partners in NIH3T3 mouse fibroblasts, we have found that S100A6 binds cofilin-1, a protein required for the ...