(1977). Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proc Natl Acad Sci U S A 74, 5613-7.Wehland, J., Osborn, M., and Weber, K. (1977): Phalloidin-induced actin polymerization in the cytoplasm of cultured cells ...
S4). Depletion of both xTalin1 and xTalin2 caused a marked reduction in actin stress fibers and FAs (Fig. 3a, middle row) compared to cells treated with control siRNA (Fig. 3a, upper row). Expression of wild-type (WT) xTalin1-EGFP rescued the xTalin1 and xTalin2 knockdown phenotype ...
Actinstressfibers–assembly,dynamicsandbiologicalrolesSariTojkander,GerganaGatevaandPekkaLappalainen*InstituteofBiotechnology,P.O.Box56,Universi..
Actin polymerization and assembly into stress fibers (SFs) is central to many cellular processes. However, how SFs form in response to the mechanical interaction of cells with their environment is not fully understood. Here we have identified Piezo2 mechanosensitive cationic channel as a transducer ...
We therefore describe a pathway involving the actin-severing proteins ADF and CFL1 in regulating the dynamic turnover of contractile actin stress fibers, and this is vital to prevent the nucleus from being damaged by actin contractility, in turn preserving cell survival and tissue homeostasis. ...
Taken together, our studies suggest that actin polymerization plays important roles in inflammasome activation by fibers via regulation of phagocytosis and/or spatial localization of inflammasome components. 展开 关键词: Actin polymerization Asbestos Inflammasome Mesothelial cells ...
Fig. 2: Inhibiting longitudinal polymerization of actin filament in axons impaired intersynaptic SV traffic. We tested the effect of a low dose of latrunculin A (0.1 µM, 3 min) (low), a G-actin buffer, on the traffic of SVs when labeled with Syt1-IgG:QDs (a, b) and on ...
Migrating rat corneal epithelial cells in tissue culture are treated with 0.1-1.0 microgram/ml cytochalasin D, an inhibitor of actin polymerization. This resulted in a generalized disruption of stress fibers, causing actin to the diffusely redistributed into discrete, round patches. Most, but not ...
cDNA-mediated overexpression of β-thymosins in CV1 fibroblasts induced extensive loss of phalloidinstained actin stress fibers. Stress fibers in the cell center were more susceptible than those at the periphery. There was a decrease in the number of focal adhesions, 展开 ...
While stress fibers are commonly observed in adherent cells, we were not able to verify that the phalloidin-positive actin filaments observed in the present study were stress fibers. The punctuated distribution of FAK and α-actinin could reflect sites of focal adhesion that were present while ...